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dc.contributor.authorDiocares, Rene
dc.contributor.authorMorey, Taylor
dc.contributor.authorHolguin, Gina
dc.date.accessioned2017-05-03T16:59:43Z
dc.date.available2017-05-03T16:59:43Z
dc.date.issued2006
dc.identifier.issn00032697
dc.identifier.doi10.1016/j.ab.2006.01.022
dc.identifier.urihttp://hdl.handle.net/10072/27875
dc.description.abstractHeavy nitrogen (15N2)1 is often used as a label for nitrogen Wxation experiments in which it is desirable to monitor directly the rate of nitrogen Wxation and the destination of the resulting metabolites in an organism or ecosystem [1-4]. Heavy nitrogen can be purchased in a pressurized cylinder or produced in the laboratory with specialized equipment, but both methods of obtaining the gas are expensive. These methods also introduce a complication because a typical nitrogen Wxation experiment requires samples of gas at atmospheric pressure. The pressure must be reduced with a pressure regulator in the case of gas in a pressurized cylinder, and traditional laboratory methods produce heavy nitrogen at pressures well below atmospheric that are not generally suitable for direct use in nitrogen Wxation experiments [1]. This is because there is no easy way in which to compress the gas to atmospheric pressure for subsequent injection into a sample bottle. This report describes a simple alternative technique that produces small quantities (limited by the volume of available plastic syringes) of heavy nitrogen at atmospheric pressure with readily available analytical chemicals, laboratory supplies, and equipment. The technique is based on that of Wood and Kennedy [4] but does not require their special apparatus.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAcademic Press
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom302
dc.relation.ispartofpageto304
dc.relation.ispartofissue2
dc.relation.ispartofjournalAnalytical Biochemistry
dc.relation.ispartofvolume352
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0601
dc.titleProducing and dispensing small quantities of 15N2 gas at atmospheric pressure
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyOther, Other
gro.date.issued2015-02-06T01:35:42Z
gro.hasfulltextNo Full Text
gro.griffith.authorDiocares, Rene


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