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dc.contributor.authorPrato, S
dc.contributor.authorMaxwell, T
dc.contributor.authorPinzon-Charry, A
dc.contributor.authorSchmidt, CW
dc.contributor.authorCorradin, G
dc.contributor.authorLopez, JA
dc.date.accessioned2017-05-03T16:58:40Z
dc.date.available2017-05-03T16:58:40Z
dc.date.issued2005
dc.date.modified2009-12-21T06:47:03Z
dc.identifier.issn0014-2980
dc.identifier.doi10.1002/eji.200425771
dc.identifier.urihttp://hdl.handle.net/10072/27881
dc.description.abstractThe circumsporozoite (CS) is the most abundant surface protein of the Plasmodium sporozoite, and is also present early in the liver stage of the infection. Following successful protective experiments in mice and monkeys, the synthetic 102-mer malaria vaccine polypeptide representing the C-terminal region of the CS of Plasmodium falciparum was tested in a clinical trial with healthy human volunteers. This vaccine induced strong CD8+, CD4+ T lymphocyte and antibody responses specific for the immunizing peptide. CD8+ T lymphocyte responses elicited in HLA-A*0201 volunteers recognized two well-defined cytotoxic T lymphocyte epitopes within the CS. Here, we show that both monocyte-derived dendritic cells (Mo-DC) and Epstein-Barr virus-transformed B-lymphoblastoid cells (LCL) can present a cytotoxic T lymphocyte epitope contained within the 102-mer synthetic peptide. Paraformaldehyde and low temperature inhibited presentation, indicating that cellular processing was required. Using specific inhibitors, we show that, in both cell types, processing requires the proteasome and the MHC class I pathway, while the endosomal compartment appears to be critical only for the presentation by Mo-DC. Antigen uptake is associated with actin polymerization in both cell types. These in vitro results demonstrate the likely pathway of antigen presentation achieved via vaccination with this synthetic peptide.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley - VCH Verlag GmbH & Co. KGaA
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom681
dc.relation.ispartofpageto689
dc.relation.ispartofissue3
dc.relation.ispartofjournalEuropean Journal of Immunology
dc.relation.ispartofvolume35
dc.rights.retentionY
dc.subject.fieldofresearchImmunology not elsewhere classified
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchcode110799
dc.subject.fieldofresearchcode1107
dc.titleMHC class I-restricted exogenous presentation of a synthetic 102-mer malaria vaccine polypeptide
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorLopez Ramirez, Alejandro
gro.griffith.authorPinzon-Charry, Alberto


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