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  • Nucleic Acid Amplification Technique for Detection of Chlamydia trachomatis Infection from Clinical Urogenital Swabs

    Author(s)
    Biroš, E.
    Bodnár, J.
    Biroš, I.
    Birošová, E.
    Mojžiš, J.
    Hrivſák, M.
    Klimčáková, L.
    Findlay, Ian
    Miroššay, A.
    Miroššay, L.
    Griffith University Author(s)
    Findlay, Ian
    Year published
    2007
    Metadata
    Show full item record
    Abstract
    An improved nucleic acid amplification test (NAAT) to detect Chlamydia trachomatis infections, based on PCR amplification within its cryptic plasmid (CT1/CT2 Test) was developed. DNA was extracted from urogenital swabs and a 594-bp long DNA fragment from the cryptic plasmid (pCT) was amplified. The sensitivity and specificity of the CT1/CT2 Test were determined to be 100 and 99 %, respectively, when directly compared with current amplification kit for sexually transmitted diseases (MPCR). Basic epidemiological data related to the patients attending gynecological and/or urological clinics are also provided. The overall ...
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    An improved nucleic acid amplification test (NAAT) to detect Chlamydia trachomatis infections, based on PCR amplification within its cryptic plasmid (CT1/CT2 Test) was developed. DNA was extracted from urogenital swabs and a 594-bp long DNA fragment from the cryptic plasmid (pCT) was amplified. The sensitivity and specificity of the CT1/CT2 Test were determined to be 100 and 99 %, respectively, when directly compared with current amplification kit for sexually transmitted diseases (MPCR). Basic epidemiological data related to the patients attending gynecological and/or urological clinics are also provided. The overall prevalence rate in this group of patients suspected for C. trachomatis infection was determined to be about 95 per 1000 (88 and 107 per 1000 in females and males, respectively). It demonstrates that the CT1/CT2 Test is suitable for epidemiological screening and/or diagnostic practice.
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    Journal Title
    Folia Microbiologica
    Volume
    52
    Issue
    4
    DOI
    https://doi.org/10.1007/BF02932100
    Subject
    Microbiology
    Medical Microbiology
    Publication URI
    http://hdl.handle.net/10072/27949
    Collection
    • Journal articles

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