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dc.contributor.authorScott, Stacyen_US
dc.contributor.authorBugarcic, Andreaen_US
dc.contributor.authorBlanchard, Helenen_US
dc.date.accessioned2017-04-24T11:30:09Z
dc.date.available2017-04-24T11:30:09Z
dc.date.issued2009en_US
dc.date.modified2009-12-23T07:13:29Z
dc.identifier.issn09298665en_US
dc.identifier.doi10.2174/092986609789071342en_AU
dc.identifier.urihttp://hdl.handle.net/10072/27989
dc.description.abstractOxidized human galectin-1 plays a role in the immune response to injured axons. Over-expression of galectin-1 by cancer, in combination with cancer associated oxidative stress suggests oxidized human galectin-1 may also play a role(s) in tumourigenesis. Here we generate milligram quantities of oxidized human galectin-1 and undertake biophysical characterization. The protein adopts a number of different states. Two separable oxidized forms are identified that exist as largely mono-disperse solutions at higher milligram/ml concentrations. The presence of disulphide bonds is confirmed for these two protein forms, as is their change in overall shape and loss of lectin activity. Our studies lead to production of a particular mono-disperse oxidized human galectin-1 species that is anticipated most optimal for investigations requiring milligram/ml concentrations such as X-ray crystallography.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherBentham Science Publishersen_US
dc.publisher.placeUSAen_US
dc.publisher.urihttp://www.bentham.org/ppl/en_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom1249en_US
dc.relation.ispartofpageto1255en_US
dc.relation.ispartofissue10en_US
dc.relation.ispartofjournalProtein and Peptide Lettersen_US
dc.relation.ispartofvolume16en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classifieden_US
dc.subject.fieldofresearchcode060199en_US
dc.titleCharacterisation of oxidized recombinant human galectin-1en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.date.issued2009
gro.hasfulltextNo Full Text


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