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dc.contributor.authorNinov, Kerli
dc.contributor.authorLedur, Monica Correa
dc.contributor.authorAlves, Helena Javiel
dc.contributor.authordo Rosario, Millor Fernandes
dc.contributor.authorNones, Katia
dc.contributor.authorCoutinho, Luiz Lehmann
dc.date.accessioned2018-01-12T02:10:16Z
dc.date.available2018-01-12T02:10:16Z
dc.date.issued2008
dc.date.modified2010-01-07T06:45:00Z
dc.identifier.issn01039016en_US
dc.identifier.doi10.1590/S0103-90162008000200016en_US
dc.identifier.urihttp://hdl.handle.net/10072/28108
dc.description.abstractLeptin, a polypeptide hormone secreted mainly by adipose tissue, plays an important role in feed intake regulation, energy metabolism and reproduction in several species. Its function has been intensively studied in mammals; however, in birds limited information is available. The cDNA sequence for chicken leptin has been reported, and high hepatic expression levels of leptin were associated with fat deposition in selected bird lines. However, controversies still remain concerning to the chicken leptin gene and several authors failed to amplify this gene from genomic DNA or cDNA. In view of this controversy and the importance of this gene, the present study aimed to investigate the leptin gene in a population of birds developed by Embrapa Swine and Poultry Research Center (Brazil). First of all, the sequences of Gallus gallus leptin gene (GenBank AF012727) and Mus musculus (GenBank NM_008493) were aligned with the objective of designing primers in conserved regions among the two species, since 94.6% of similarity is described in the literature in those species. For all four pairs of primers designed, several amplification tests were performed with both DNA and cDNA, but neither unique fragment nor expected band size was ever achieved. The leptin sequence in GenBank does not represent the sequence of the chicken leptin gene.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherUniversidade de Sao Pauloen_US
dc.publisher.placeBrazilen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom214en_US
dc.relation.ispartofpageto219en_US
dc.relation.ispartofissue2en_US
dc.relation.ispartofjournalScientia Agricolaen_US
dc.relation.ispartofvolume65en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchAgricultural Biotechnology not elsewhere classifieden_US
dc.subject.fieldofresearchcode100199en_US
dc.titleInvestigation of Leptin gene in broiler and layer chicken linesen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dcterms.licensehttps://creativecommons.org/licenses/by-nc/4.0/deed.enen_US
dc.description.versionPublisheden_US
gro.rights.copyright© 2008. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.en_US
gro.date.issued2008
gro.hasfulltextFull Text


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