Show simple item record

dc.contributor.authorM. Vitale, Alejandra
dc.contributor.authorM. Gonzalez, Olga
dc.contributor.authorParborell, Fernanda
dc.contributor.authorIrusta, Griselda
dc.contributor.authorCampo, Stella
dc.contributor.authorTesone, Marta
dc.date.accessioned2017-05-03T16:57:38Z
dc.date.available2017-05-03T16:57:38Z
dc.date.issued2002
dc.date.modified2010-01-11T07:55:42Z
dc.identifier.issn00063363
dc.identifier.doi10.1095/biolreprod.102.003673
dc.identifier.urihttp://hdl.handle.net/10072/28201
dc.description.abstractThe purpose of this study was to evaluate the role of inhibin A in follicular development and apoptosis-related mechanisms in preantral and early antral follicles from prepubertal diethylstilbestrol (DES)-treated rats. Granulosa cells isolated from the ovaries of 23- to 25-day-old rats were cultured in serum-free medium containing FSH (20 ng/ml), transforming growth factor ߠ(5 ng/ml), and estradiol (50 ng/ml) in the presence or absence of different concentrations of recombinant human inhibin A. 3H-Thymidine incorporation was decreased in the presence of Inh, but no significant changes were observed in progesterone and estradiol levels in culture medium. An increase in low molecular weight DNA fragmentation indicative of apoptosis and an increase in the levels of Bax protein with no changes in Bcl-2 protein levels were evident in early antral follicles incubated for 24 h with Inh. For each animal, Inh (0.5 姯ovary) was injected intrabursally in one ovary, and the contralateral ovary served as a control. Ovarian histology revealed an inhibitory effect of Inh treatment on the follicular development induced by DES. At 24 h after Inh injection, the number of preantral follicles was increased compared with controls, whereas the number of early antral follicles was decreased. In addition, in vivo Inh treatment caused an increase in the percentage of apoptotic cells in preantral and early antral follicles. These results suggest that inhibin produced by the dominant follicle may act as a paracrine factor inhibiting the growth of neighboring follicles, thus participating in the mechanism of follicular selection.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSociety for the Study of Reproduction
dc.publisher.placeUSA
dc.relation.ispartofpagefrom1989
dc.relation.ispartofpageto1995
dc.relation.ispartofissue6
dc.relation.ispartofjournalBiology of Reproduction
dc.relation.ispartofvolume67
dc.subject.fieldofresearchCell Development, Proliferation and Death
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode060103
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode11
dc.titleInhibin A Increases Apoptosis in Early Ovarian Antral Follicles of Diethylstilbestrol-Treated Rats
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2002
gro.hasfulltextNo Full Text
gro.griffith.authorVitale, Alejandra M.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record