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dc.contributor.authorLambert, D.en_US
dc.contributor.authorBaker, A.en_US
dc.contributor.authorHuynen, L.en_US
dc.contributor.authorHaddrath, O.en_US
dc.contributor.authorHebert, P.en_US
dc.contributor.authorMillar, C.en_US
dc.date.accessioned2017-05-03T14:39:01Z
dc.date.available2017-05-03T14:39:01Z
dc.date.issued2005en_US
dc.date.modified2010-01-14T07:05:49Z
dc.identifier.issn0022-1503en_US
dc.identifier.doi10.1093/jhered/esi035en_AU
dc.identifier.urihttp://hdl.handle.net/10072/28282
dc.description.abstractA complete DNA-based inventory of the Earth's present biota using large-scale high-throughput DNA sequencing of signature region(s) (DNA barcoding) is an ambitious proposal rivaling the Human Genome Project. We examine whether this approach will also enable us to assess the past diversity of the earth's biota. To test this, we sequenced the 5' terminus of the mitochondrial cytochrome c oxidase I (COI) gene of individuals belonging to a group of extinct ratite birds, the moa of New Zealand. Moa comprised a large number of taxa that radiated in isolation on this oceanic landmass. Using a phylogenetic approach based on a large data set including protein coding and 12S DNA sequences as well as morphology, we now have precise information about the number of moa species that once existed. We show that each of the moa species detected using this extensive data set has a unique COI barcode(s) and that they all show low levels of within-species COI variation. Consequently, we conclude that COI sequences accurately identify the species discovered using the larger data set. Hence, more generally, this study suggests that DNA barcoding might also help us detect other extinct animal species and that a large-scale inventory of ancient life is possible.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherOxford University Pressen_US
dc.publisher.placeUKen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom279en_US
dc.relation.ispartofpageto284en_US
dc.relation.ispartofissue3en_US
dc.relation.ispartofjournalJournal of Heredityen_US
dc.relation.ispartofvolume96en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMolecular Evolutionen_US
dc.subject.fieldofresearchcode060409en_US
dc.titleIs a large-scale DNA-based inventory of ancient life possible?en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2005
gro.hasfulltextNo Full Text


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