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dc.contributor.authorMueller, C Catharina
dc.contributor.authorNourse, Jamie P
dc.contributor.authorNguyen, Tam H
dc.contributor.authorCrane, Denis I
dc.date.accessioned2017-05-03T11:01:59Z
dc.date.available2017-05-03T11:01:59Z
dc.date.issued2009
dc.date.modified2010-06-24T05:17:22Z
dc.identifier.issn0165-0270
dc.identifier.doi10.1016/j.jneumeth.2009.04.025
dc.identifier.urihttp://hdl.handle.net/10072/28509
dc.description.abstractThe Cre/loxP-system has become an invaluable tool for the generation of tissue-specific gene disruption in mice. However, because Cre recombinase excision of individual genes can be variable, an accurate and sensitive method is necessary to determine the ultimate level of gene disruption. The analysis of gene disruption is particularly difficult for tissue that has been fixed for (immuno)histochemical analysis with paraformaldehyde. Here, we describe a simple, rapid and cost effective method for measurement of gene disruption using quantitative real-time PCR, through application to the analysis of PEX13 gene disruption in a brain-specific PEX13 mouse mutant. We show that this general protocol is suitable for both normal and paraformaldehyde-fixed tissue.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeAmsterdam, Netherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom73
dc.relation.ispartofpageto81
dc.relation.ispartofjournalJournal of Neuroscience Methods
dc.relation.ispartofvolume181
dc.rights.retentionY
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchNeurosciences not elsewhere classified
dc.subject.fieldofresearchCognitive and computational psychology
dc.subject.fieldofresearchcode3209
dc.subject.fieldofresearchcode320999
dc.subject.fieldofresearchcode5204
dc.titleQuantitative genotyping of mouse brain-specific PEX13 gene disruption by real-time PCR
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2009
gro.hasfulltextNo Full Text
gro.griffith.authorCrane, Denis I.


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