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dc.contributor.authorLogue, Carie-Anne
dc.contributor.authorPeak, Ian RA
dc.contributor.authorBeacham, Ifor R
dc.date.accessioned2017-05-03T12:19:21Z
dc.date.available2017-05-03T12:19:21Z
dc.date.issued2009
dc.date.modified2010-01-29T06:18:32Z
dc.identifier.issn0167-7012
dc.identifier.doi10.1016/j.mimet.2008.12.007
dc.identifier.urihttp://hdl.handle.net/10072/28519
dc.description.abstractBurkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent363772 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom320
dc.relation.ispartofpageto323
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of Microbiological Methods
dc.relation.ispartofvolume76
dc.rights.retentionY
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchInfectious agents
dc.subject.fieldofresearchImmunology not elsewhere classified
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchMedical bacteriology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310702
dc.subject.fieldofresearchcode320499
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode320701
dc.titleFacile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2009 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2009
gro.hasfulltextFull Text
gro.griffith.authorBeacham, Ifor R.
gro.griffith.authorPeak, Ian


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