dc.contributor.author | Logue, Carie-Anne | |
dc.contributor.author | Peak, Ian RA | |
dc.contributor.author | Beacham, Ifor R | |
dc.date.accessioned | 2017-05-03T12:19:21Z | |
dc.date.available | 2017-05-03T12:19:21Z | |
dc.date.issued | 2009 | |
dc.date.modified | 2010-01-29T06:18:32Z | |
dc.identifier.issn | 0167-7012 | |
dc.identifier.doi | 10.1016/j.mimet.2008.12.007 | |
dc.identifier.uri | http://hdl.handle.net/10072/28519 | |
dc.description.abstract | Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 363772 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.publisher.place | Netherlands | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 320 | |
dc.relation.ispartofpageto | 323 | |
dc.relation.ispartofissue | 3 | |
dc.relation.ispartofjournal | Journal of Microbiological Methods | |
dc.relation.ispartofvolume | 76 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Microbiology | |
dc.subject.fieldofresearch | Infectious agents | |
dc.subject.fieldofresearch | Immunology not elsewhere classified | |
dc.subject.fieldofresearch | Medical microbiology | |
dc.subject.fieldofresearch | Medical bacteriology | |
dc.subject.fieldofresearchcode | 3107 | |
dc.subject.fieldofresearchcode | 310702 | |
dc.subject.fieldofresearchcode | 320499 | |
dc.subject.fieldofresearchcode | 3207 | |
dc.subject.fieldofresearchcode | 320701 | |
dc.title | Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2009 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. | |
gro.date.issued | 2009 | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Beacham, Ifor R. | |
gro.griffith.author | Peak, Ian | |