Show simple item record

dc.contributor.authorLogue, Carie-Anneen_US
dc.contributor.authorPeak, Ianen_US
dc.contributor.authorBeacham, Iforen_US
dc.date.accessioned2017-05-03T12:19:21Z
dc.date.available2017-05-03T12:19:21Z
dc.date.issued2009en_US
dc.date.modified2010-01-29T06:18:32Z
dc.identifier.issn0167-7012en_US
dc.identifier.doi10.1016/j.mimet.2008.12.007en_AU
dc.identifier.urihttp://hdl.handle.net/10072/28519
dc.description.abstractBurkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.format.extent363772 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom320en_US
dc.relation.ispartofpageto323en_US
dc.relation.ispartofissue3en_US
dc.relation.ispartofjournalJournal of Microbiological Methodsen_US
dc.relation.ispartofvolume76en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchInfectious Agentsen_US
dc.subject.fieldofresearchImmunology not elsewhere classifieden_US
dc.subject.fieldofresearchMedical Bacteriologyen_US
dc.subject.fieldofresearchcode060502en_US
dc.subject.fieldofresearchcode110799en_US
dc.subject.fieldofresearchcode110801en_US
dc.titleFacile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolatesen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2009 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_AU
gro.date.issued2009
gro.hasfulltextFull Text


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record