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dc.contributor.authorLeusch, Fredericen_US
dc.contributor.authorR. van den Heuvel, Michaelen_US
dc.contributor.authorChapman, Heatheren_US
dc.contributor.authorRavi Gooneratne, S.en_US
dc.contributor.authorM.E. Eriksson, Annaen_US
dc.contributor.authorA. Tremblay, Louisen_US
dc.date.accessioned2017-04-24T14:56:45Z
dc.date.available2017-04-24T14:56:45Z
dc.date.issued2006en_US
dc.date.modified2010-07-08T08:09:28Z
dc.identifier.issn15320456en_US
dc.identifier.doi10.1016/j.cbpc.2005.12.010en_AU
dc.identifier.urihttp://hdl.handle.net/10072/28768
dc.description.abstractChemicals released into the environment by anthropogenic activities have been linked to estrogenic or androgenic effects in exposed wildlife, and there is a need to develop and validate rapid and cost-effective methods to quantify the total estrogenic and androgenic activity of environmental water samples. In this study, estrogen receptors (ER) were isolated from sheep (Ovis aries) uteri and rainbow trout (Oncorhynchus mykiss) livers and androgen receptors (AR) were isolated from rainbow trout brains. The isolated receptors were used in competitive receptor binding assays to test the affinity of known estrogenic and androgenic chemicals for the receptor binding site, and results were compared with literature values for the rat uterine ER binding assay and the E-Screen. The relative binding affinities of the tested compounds to ER from different species were similar, and binding to the ER was a more responsive endpoint than the cellular effect measured in the E-Screen. Using the sheep ER binding assay in combination with solid-phase extraction, the estrogenic activity in a raw sewage sample from a municipal treatment plant in Brisbane (Queensland, Australia) was measured at 51-73 ng/L estradiol equivalents (EEq).en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeNew York, United Statesen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom117en_US
dc.relation.ispartofpageto126en_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalComparative Biochemistry and Physiology, Part Cen_US
dc.relation.ispartofvolume143en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMulti-Disciplinaryen_US
dc.subject.fieldofresearchcode999999en_US
dc.titleDevelopment of methods for extraction and in vitro quantification of estrogenic and androgenic activity of wastewater samplesen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Sciences, Griffith School of Environmenten_US
gro.date.issued2006
gro.hasfulltextNo Full Text


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