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  • Intrinsic and extrinsic P2 purinoceptor-mediated cardioprotection in mice

    Author(s)
    Headrick, John P
    Wee, Shirley
    Peart, Jason N
    Griffith University Author(s)
    Headrick, John P.
    Wee, Shirley S.
    Peart, Jason N.
    Year published
    2007
    Metadata
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    Abstract
    P2 purinoceptor-dependent control of resistance to ischemia reperfusion injuries was studied in Langendorff perfused C57/Bl6 mouse hearts subjected to 20 min ischemia and 45 min reperfusion. Effects of P2 agonism and antagonism were assessed. Control hearts recovered 68 ᠴ mm Hg ventricular pressure (63 ᠳ% pre-ischaemia), exhibited sustained diastolic contracture (23 ᠲ mm Hg), and released 26 ᠴ U/g lactate dehydrogenase (LDH) during reperfusion, evidencing oncosis. Treatment with 250 nM of the P2 agonist uridine 5'-triphosphate (UTP) for 10 min prior to and 10 min after ischemia reduced diastolic contracture by not, vert, ...
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    P2 purinoceptor-dependent control of resistance to ischemia reperfusion injuries was studied in Langendorff perfused C57/Bl6 mouse hearts subjected to 20 min ischemia and 45 min reperfusion. Effects of P2 agonism and antagonism were assessed. Control hearts recovered 68 ᠴ mm Hg ventricular pressure (63 ᠳ% pre-ischaemia), exhibited sustained diastolic contracture (23 ᠲ mm Hg), and released 26 ᠴ U/g lactate dehydrogenase (LDH) during reperfusion, evidencing oncosis. Treatment with 250 nM of the P2 agonist uridine 5'-triphosphate (UTP) for 10 min prior to and 10 min after ischemia reduced diastolic contracture by not, vert, similar 50% (9 ᠲ mm Hg), improved pressure development (85 ᠵ mm Hg; 77 ᠲ% of baseline), and reduced LDH loss by 60% (11 ᠲ U/g). In contrast, P2Y1 agonism with 50 nM 2-methyl-thio-ATP was ineffective. P2 antagonism with 200 占suramin impaired post-ischemic outcomes, exaggerating contractile dysfunction (41 ᠲ mm Hg diastolic pressure; 33 ᠵ mm Hg developed pressure) and LDH loss (53 ᠹ U/g). In the presence of suramin, UTP no longer modified post-ischemic function or oncosis. Evidence of intrinsic P2-mediated protection is consistent with ischemic elevations in microdialysate [UTP], [ATP] and [ADP]. In summary, these data evidence myocardial protection via endogenous and exogenous P2 agonists: enhanced recoveries with UTP, together with exaggeration of intrinsic injury and inhibition of UTP-protection with suramin, support protection via exogenously and intrinsically activated P2 purinoceptors. Data implicate P2Y2 receptors in cardioprotection, though further work is required to identify the sub-type involved.
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    Journal Title
    Journal of Molecular and Cellular Cardiology
    Volume
    42
    Issue
    6 Supp1
    DOI
    https://doi.org/10.1016/j.yjmcc.2007.03.572
    Subject
    Cardiovascular medicine and haematology
    Cardiology (incl. cardiovascular diseases)
    Medical physiology
    Publication URI
    http://hdl.handle.net/10072/28910
    Collection
    • Journal articles

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