The expression of chicken interleukin 2 in Pichia yeast
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Objective To express recombinant chicken interleukin 2 ( IL22) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL22. Methods Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL22 gene was amplified and cloned into pPIC9. The pPIC92IL22 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Result s The protein was examined with SDS2PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion The recombinant protein of chicken IL22 has been expressed in Pichia yeast .
Journal of Xi'an Jiaotong University (Medical Sciences)
Biomedical Engineering not elsewhere classified