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dc.contributor.authorJian, Xuen_US
dc.contributor.authorFan, Yongjunen_US
dc.contributor.authorLong, Lien_US
dc.contributor.authorWangjun, Wanen_US
dc.contributor.authorJianrong, Lien_US
dc.contributor.authorLian, Yuen_US
dc.date.accessioned2017-05-03T13:54:40Z
dc.date.available2017-05-03T13:54:40Z
dc.date.issued2004en_US
dc.date.modified2010-02-26T06:37:21Z
dc.identifier.issn16718259en_US
dc.identifier.urihttp://hdl.handle.net/10072/28971
dc.description.abstractObjective To express recombinant chicken interleukin 2 ( IL22) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL22. Methods Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL22 gene was amplified and cloned into pPIC9. The pPIC92IL22 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Result s The protein was examined with SDS2PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion The recombinant protein of chicken IL22 has been expressed in Pichia yeast .en_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherXi'an Jiaotong Daxueen_US
dc.publisher.placeChinaen_US
dc.publisher.urihttp://en.cnki.com.cn/Article_en/CJFDTOTAL-XAYX200404008.htmen_AU
dc.relation.ispartofpagefrom339en_US
dc.relation.ispartofpageto342en_US
dc.relation.ispartofissue4en_US
dc.relation.ispartofjournalJournal of Xi'an Jiaotong University (Medical Sciences)en_US
dc.relation.ispartofvolume25en_US
dc.subject.fieldofresearchBiomedical Engineering not elsewhere classifieden_US
dc.subject.fieldofresearchcode090399en_US
dc.titleThe expression of chicken interleukin 2 in Pichia yeasten_US
dc.typeJournal articleen_US
dc.type.descriptionC2 - Non HERDC Eligibleen_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2004
gro.hasfulltextNo Full Text


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