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dc.contributor.authorF. Young, Sharla
dc.contributor.authorSchwartz, Jeffrey
dc.contributor.authorRose, James C.
dc.date.accessioned2017-05-03T11:46:05Z
dc.date.available2017-05-03T11:46:05Z
dc.date.issued2003
dc.date.modified2010-03-05T06:13:46Z
dc.identifier.issn19337191
dc.identifier.doi10.1016/S1071-55760300008-X
dc.identifier.urihttp://hdl.handle.net/10072/29084
dc.description.abstractObjective: Previous studies have shown that anterior pituitary expression of corticotropin-releasing hormone type 1 receptor (GRH-R1) decreases during late gestation. This study sought to determine whether this reduction is from a decrease in the number of cells expressing CRH receptors of a decrease in the number of CRH receptors per cell. Methods: Fetuses were studied at 100 days' gestational age (100 dGA), 120 dGA, or 140 dGA. Dispersed anterior pituitary cells were incumbated with or without fluorescein-conjugated CRH (FL-CRH). FL-CRH binding was assessed by flow cytometry. Results: The number of CRH receptors per cell decreased at 120 dGA and 140 dGA. However, because of an increase in the percentage of cells expressing CRH receptors at 120 dGA, the total receptor index was similar at 100 dGA and 120 dGA. The receptor index tended to decrease at 140 dGA. Conclusion: FL-CRH binding was decreased at 140 dGA due to a decrease in the percentage of cells binding CRH and the number of CRH receptors per cell. This could contribute to the decrease in CRH-stimulated adrenocorticotropin release during late gestation in fetal sheep.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSage
dc.publisher.placeUSA
dc.relation.ispartofpagefrom130
dc.relation.ispartofpageto135
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of the Society for Gynecologic Investigation
dc.relation.ispartofvolume10
dc.subject.fieldofresearchPaediatrics and Reproductive Medicine not elsewhere classified
dc.subject.fieldofresearchPaediatrics and Reproductive Medicine
dc.subject.fieldofresearchcode111499
dc.subject.fieldofresearchcode1114
dc.titleOntogeny of Corticotropin-Releasing Hormone Binding in Anterior Pituitaries of Fetal Sheep
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorSchwartz, Jeffrey


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