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dc.contributor.authorHolmes, Roger S
dc.contributor.authorCox, Laura A
dc.contributor.authorVandeBerg, John L
dc.date.accessioned2017-05-03T16:58:29Z
dc.date.available2017-05-03T16:58:29Z
dc.date.issued2009
dc.date.modified2010-06-25T06:42:35Z
dc.identifier.issn1744-117X
dc.identifier.doi10.1016/j.cbd.2009.03.002
dc.identifier.urihttp://hdl.handle.net/10072/29363
dc.description.abstractMammalian carboxylesterases (CES) exhibit broad substrate specificities, catalyse hydrolytic and transesterification reactions with a wide range of drugs and xenobiotics and are widely distributed in the body. Four CES classes have been previously described, namely CES1 (major liver form); CES2 (major intestinal form); CES3 (highest activity in the colon); and CES5, a secreted enzyme found in mammalian kidney and male reproductive fluids. In silico methods were used to predict the amino acid sequences, structures and gene locations for a new class of CES genes and proteins, designated as CES6. Mammalian CES6 amino acid sequence alignments and predicted secondary and tertiary structures enabled the identification of key CES sequences previously reported for human CES1, but with CES6 specific sequences and properties: high isoelectric points (pI values of 8.8-9.4 compared with 5.4-6.2 for human CES1, CES2, CES3 and CES5); being predicted for secretion into body fluids compared with human CES1, human CES2 and CES3, which are membrane bound; and having Asn or Glu residues at the predicted CES1 Z-site for which a Gly residue plays a major role in cholesterol binding. Mammalian CES6 genes are located in tandem with CES2 and CES3 genes, are transcribed on the positive DNA strand and contain 14 exons. Human and mouse CES6-like transcripts have been previously reported to be widely distributed in the body but are localized in specific regions of the brain, including the cerebellum. CES6 may play a role in the detoxification of drugs and xenobiotics in neural and other tissues of the body and in the cerebrospinal fluid.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.publisher.urihttp://www.sciencedirect.com/science/journal/1744117X
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom209
dc.relation.ispartofpageto217
dc.relation.ispartofissue3
dc.relation.ispartofjournalComparative Biochemistry and Physiology, Part D
dc.relation.ispartofvolume4
dc.rights.retentionY
dc.subject.fieldofresearchGenomics
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchcode060408
dc.subject.fieldofresearchcode0604
dc.titleA new class of mammalian carboxylesterase CES6
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2009
gro.hasfulltextNo Full Text
gro.griffith.authorHolmes, Roger S.


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