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dc.contributor.authorKaity, Adam
dc.contributor.authorAshmore, SE
dc.contributor.authorDrew, RA
dc.date.accessioned2017-05-03T11:15:34Z
dc.date.available2017-05-03T11:15:34Z
dc.date.issued2009
dc.date.modified2010-08-24T06:52:10Z
dc.identifier.issn0721-7714
dc.identifier.doi10.1007/s00299-009-0742-y
dc.identifier.urihttp://hdl.handle.net/10072/29503
dc.description.abstractThis paper is the first report of field performance and evaluation of morphological traits following cryopreservation in four genotypes of Carica papaya (Z6, 97, TS2 and 35). It also describes the successful establishment of in vitro plantlets following vitrification-based cryopreservation of shoot tips and their acclimatisation through to field establishment. Cloned plants resulting from untreated controls, as well as controls taken at three other stages of the cryopreservation process (dissection, pre-treatment, plant vitrification solution 2 (PVS2) treatment) and cryopreserved plants were established to ensure a rigorous appraisal of any variation. Results indicate no differences between any of the control plants or cryopreserved plants for either growth performance or morphology. In addition, both randomly amplified DNA fingerprinting and amplified DNA methylation polymorphism markers were used to assess any genomic or methylation changes in genotype 97 at four different developmental stages post cryopreservation (in vitro, acclimatisation and field). Only small genomic DNA modifications (0-8.3%) were detected in field stage plants and methylation modifications (0-4.3%) were detected at both the in vitro and field stages for samples treated with PVS2 or cryopreservation.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom1421
dc.relation.ispartofpageto1430
dc.relation.ispartofjournalPlant Cell Reports
dc.relation.ispartofvolume28
dc.rights.retentionY
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchPlant biology not elsewhere classified
dc.subject.fieldofresearchCrop and pasture production
dc.subject.fieldofresearchHorticultural production not elsewhere classified
dc.subject.fieldofresearchcode3108
dc.subject.fieldofresearchcode310899
dc.subject.fieldofresearchcode3004
dc.subject.fieldofresearchcode300899
dc.titleField Performance Evaluation and Genetic Integrity Assessment of Cryopreserved Papaya Clones
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2009
gro.hasfulltextNo Full Text
gro.griffith.authorDrew, Roderick A.


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