dc.contributor.author | Bei, Weijian | |
dc.contributor.author | Zang, Linquan | |
dc.contributor.author | Guo, Jiao | |
dc.contributor.author | Peng, Wenlie | |
dc.contributor.author | Xu, Anlong | |
dc.contributor.author | Good, David A | |
dc.contributor.author | Hu, Yinming | |
dc.contributor.author | Wu, Wei | |
dc.contributor.author | Hu, Dehui | |
dc.contributor.author | Zhu, Xinghong | |
dc.contributor.author | Wei, Ming | |
dc.contributor.author | Li, Chuyuan | |
dc.date.accessioned | 2017-05-03T13:02:12Z | |
dc.date.available | 2017-05-03T13:02:12Z | |
dc.date.issued | 2009 | |
dc.date.modified | 2010-06-24T05:22:05Z | |
dc.identifier.issn | 0378-8741 | |
dc.identifier.doi | 10.1016/j.jep.2009.07.034 | |
dc.identifier.uri | http://hdl.handle.net/10072/29512 | |
dc.description.abstract | Ethnopharmacological relevance: Flavonoids, extracted from the leaves of Diospyros kaki, are the main therapeutic components of NaoXingQing (NXQ), a potent and patented Chinese herbal remedy widely used in China for the treatment of apoplexy syndrome. Aim of the study: To investigate the neuroprotective effects of FLDK-P70, a standardized flavonoid extract, using in vivo rat models of both focal ischemia/reperfusion (I/R) injury induced by middle cerebral artery occlusion (MCAO) and on transient global brain ischemia induced by four-vessel occlusion (4-VO). We also aim to examine the effects of FLDK-P70 on glutamate-induced cell injury of hippocampal neurons as well as on hypoxia-induced injury of cortical neurons in primary cell culture. Materials and methods and results: Administration of FLDK-P70 for 12 days (40, 80 mg/kg body weight, p.o., 5 days before and 7 days after 4-VO) increased the survival of hippocampal CA1 pyramidal neurons after transient global brain ischemia. Similarly, administration of FLDK-P70 for 7 days (40, 80 mg/kg body weight, p.o., 3 days before and 4 days after MCAO) significantly reduced the lesion of the insulted brain hemisphere and improved the neurological behavior of rats. In primary rat hippocampal neuronal cultures, pretreatment with FLDK-P70 (5, 10 g/ml) protected neurons from glutamate-induced excitotoxic neuronal death in a dose-dependent manner. In primary rat cerebral cortical neuronal culture, pretreatment with FLDK-P70 (25, 100 g/ml) also reduced hypoxia-reoxygen induced neuronal death and apoptosis in a dose-dependent manner. Conclusions: These in vivo and in vitro results suggest that FLDK-P70 significantly protects rats fromMCAO and 4-VO ischemic injury in vivo and protects hippocampal neurons from glutamate-induced excitotoxic injury as well as cortical neurons from hypoxia-induced injury in vitro. The mechanisms of these effects may be due to the antioxidative activity of the flavonoids. These results convincingly demonstrate that FLDK-P70 may be useful for the prevention and treatment of ischemia/reperfusion injury and other related neurodegenerative diseases. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.publisher.place | Ireland | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 134 | |
dc.relation.ispartofpageto | 142 | |
dc.relation.ispartofjournal | Journal of Ethnopharmacology | |
dc.relation.ispartofvolume | 126 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Plant biology | |
dc.subject.fieldofresearch | Traditional, complementary and integrative medicine | |
dc.subject.fieldofresearch | Traditional Chinese medicine and treatments | |
dc.subject.fieldofresearch | Pharmacology and pharmaceutical sciences | |
dc.subject.fieldofresearchcode | 3108 | |
dc.subject.fieldofresearchcode | 4208 | |
dc.subject.fieldofresearchcode | 420803 | |
dc.subject.fieldofresearchcode | 3214 | |
dc.title | Neuroprotective effects of a standardized flavonoid extract from Diospyros kaki leaves | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2009 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Wei, Ming Q. | |