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  • Zinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators

    Author(s)
    Gauci, Victoria J
    Beckhouse, Anthony G
    Lyons, Victoria
    Beh, Eric J
    Rogers, Peter J
    Dawes, Ian W
    Higgins, Vincent J
    Griffith University Author(s)
    Beckhouse, Anthony G.
    Year published
    2009
    Metadata
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    Abstract
    During the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a 'sluggish' or a 'stuck' ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis ...
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    During the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a 'sluggish' or a 'stuck' ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis identified the stress response element (STRE) motif, recognized by Msn2/4p, and was significantly enriched in the promoters of genes induced by zinc deficiency. An investigation using genome-wide analyses revealed a distinct regulon consisting of STREcontaining genes whose zinc-responsive expression was abolished in an msn2 msn4 double mutant. An STRE-driven lacZ reporter construct confirmed that expression of the genes within this regulon was perturbed by the deletion of MSN2 and MSN4 and also implicated Hog1p as a contributing factor. This research provides a better understanding of the molecular mechanisms involved in the yeast response to zinc deficiency during fermentation.
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    Journal Title
    FEMS Yeast Research
    Volume
    9
    Issue
    8
    Publisher URI
    http://www.wiley.com/bw/journal.asp?ref=1567-1356
    DOI
    https://doi.org/10.1111/j.1567-1364.2009.00557.x
    Subject
    Biological sciences
    Genomics
    Engineering
    Publication URI
    http://hdl.handle.net/10072/29828
    Collection
    • Journal articles

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