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dc.contributor.authorGauci, Victoria J
dc.contributor.authorBeckhouse, Anthony G
dc.contributor.authorLyons, Victoria
dc.contributor.authorBeh, Eric J
dc.contributor.authorRogers, Peter J
dc.contributor.authorDawes, Ian W
dc.contributor.authorHiggins, Vincent J
dc.date.accessioned2017-05-03T15:18:39Z
dc.date.available2017-05-03T15:18:39Z
dc.date.issued2009
dc.date.modified2010-05-25T07:49:29Z
dc.identifier.issn1567-1356
dc.identifier.doi10.1111/j.1567-1364.2009.00557.x
dc.identifier.urihttp://hdl.handle.net/10072/29828
dc.description.abstractDuring the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a 'sluggish' or a 'stuck' ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis identified the stress response element (STRE) motif, recognized by Msn2/4p, and was significantly enriched in the promoters of genes induced by zinc deficiency. An investigation using genome-wide analyses revealed a distinct regulon consisting of STREcontaining genes whose zinc-responsive expression was abolished in an msn2 msn4 double mutant. An STRE-driven lacZ reporter construct confirmed that expression of the genes within this regulon was perturbed by the deletion of MSN2 and MSN4 and also implicated Hog1p as a contributing factor. This research provides a better understanding of the molecular mechanisms involved in the yeast response to zinc deficiency during fermentation.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherBlackwell Publishing Ltd.
dc.publisher.placeUnited Kingdom
dc.publisher.urihttp://www.wiley.com/bw/journal.asp?ref=1567-1356
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1187
dc.relation.ispartofpageto1195
dc.relation.ispartofissue8
dc.relation.ispartofjournalFEMS Yeast Research
dc.relation.ispartofvolume9
dc.rights.retentionY
dc.subject.fieldofresearchGenomics
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchTechnology
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchcode060408
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode10
dc.subject.fieldofresearchcode09
dc.titleZinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2009
gro.hasfulltextNo Full Text
gro.griffith.authorBeckhouse, Anthony G.


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