The synthesis of biotinylated carbohydrates as probes for carbohydrate-recognizing proteins
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The intimate involvement of carbohydrate-protein interactions in a number of important biological processes has prompted several research efforts towards developing new methods of investigating these glycobiological interactions. Biotinylated oligosaccharides are emerging as a new and powerful tool in this area of research, primarily due to their high affinity towards streptavidin and their ease of immobilization on matrices. Here we describe a novel synthetic approach towards biotinylated saccharides which incorporate a UV absorbing group into the final compounds. The synthetic strategy described is applicable to a variety of saccharides, with examples of biotinylated mono-, di-, and trisaccharides being prepared with overall high efficiency.
Bioorganic & Medicinal Chemistry
Copyright 2000 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.