Show simple item record

dc.contributor.authorMutalik, S
dc.contributor.authorHewavitharana, AK
dc.contributor.authorShaw, PN
dc.contributor.authorAnissimov, YG
dc.contributor.authorRoberts, MS
dc.contributor.authorParekh, HS
dc.date.accessioned2017-05-03T15:13:55Z
dc.date.available2017-05-03T15:13:55Z
dc.date.issued2009
dc.date.modified2010-09-02T06:51:47Z
dc.identifier.issn1570-0232
dc.identifier.doi10.1016/j.jchromb.2009.08.039
dc.identifier.urihttp://hdl.handle.net/10072/30702
dc.description.abstractThe aim of the present work was to develop and validate a simple RP-HPLC method with UV detection to quantify peptide dendrimers in skin permeation experiments. Six dendrimers of varying positive charges (4+, 8+ and 16+) containing either histidine or arginine as terminal aminoacids were prepared by solid phase peptide synthesis. Mobile phase containing 0.02% (v/v) heptafluorobutyric acid in 90% acetonitrile-water was capable of separating all dendrimers from interfering peaks of receptor fluid. For the calibration of each dendrimer, a different dendrimer from the same class was selected as the internal standard. The results of preliminary human skin permeation studies showed that the developed analytical method can be successfully used for the quantification of cationic poly(aminoacid)-based dendrimers in skin permeation experiments.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent327460 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom3556
dc.relation.ispartofpageto3562
dc.relation.ispartofissue29
dc.relation.ispartofjournalJournal of Chromatography B
dc.relation.ispartofvolume877
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchPharmaceutical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3214
dc.subject.fieldofresearchcode321405
dc.titleDevelopment and validation of a reversed-phase high-performance liquid chromatographic method for quantification of peptide dendrimers in human skin permeation experiments
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2009 Elsevier B.V.. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2009
gro.hasfulltextFull Text
gro.griffith.authorAnissimov, Yuri G.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record