• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Cellular nicotinamide adenine dinucleotide (NADH) as an indicator of bacterial metabolic activity dynamics in activated sludge

    Author(s)
    Wos, ML
    Pollard, PC
    Griffith University Author(s)
    Pollard, Peter C.
    Year published
    2009
    Metadata
    Show full item record
    Abstract
    In this study, native fluorescent nicotinamide adenine dinucleotide (NADH) was used as a direct indicator of bacterial metabolic activity in activated sludge. Specific NADH concentration was dynamic and varied between 106-108 molecules per bacterial cell. Low concentrations (106-107 NADH molecules cell-1) indicate efficient bacterial metabolic activity while high concentrations (107-108 NADH molecules cell-1) indicate inefficient bacterial metabolic activity. Specific [NADH] did not correlate to changes in dissolved organic carbon, but increases correlated to decreases in oxygen uptake rates. Perhaps a lack of oxygen as the ...
    View more >
    In this study, native fluorescent nicotinamide adenine dinucleotide (NADH) was used as a direct indicator of bacterial metabolic activity in activated sludge. Specific NADH concentration was dynamic and varied between 106-108 molecules per bacterial cell. Low concentrations (106-107 NADH molecules cell-1) indicate efficient bacterial metabolic activity while high concentrations (107-108 NADH molecules cell-1) indicate inefficient bacterial metabolic activity. Specific [NADH] did not correlate to changes in dissolved organic carbon, but increases correlated to decreases in oxygen uptake rates. Perhaps a lack of oxygen as the terminal electron acceptor prevented efficient reoxidization of NADH to NAD+, which resulted in an accumulation of NADH within the cells. Also, significant amounts of NADH were released and accumulated into the extracellular medium of metabolically active E. coli cells in log phase. Such overflow metabolism may be the product of favourable conditions. Thus, the flux of both specific intracellular and extracellular [NADH] indicates the dynamics of bacterial metabolic activity in activated sludge.
    View less >
    Journal Title
    Water Science and Technology
    Volume
    60
    Issue
    3
    Publisher URI
    https://iwaponline.com/wst/article/60/3/783/15651/Cellular-nicotinamide-adenine-dinucleotide-NADH-as?searchresult=1
    DOI
    https://doi.org/10.2166/wst.2009.393
    Subject
    Environmental Monitoring
    Publication URI
    http://hdl.handle.net/10072/30943
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E
    • TEQSA: PRV12076

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander