Sialic acid dependence in rotavirus host cell invasion
Author(s)
Haselhorst, Thomas
Fleming, Fiona E
Dyason, Jeffrey C
Hartnell, Regan D
Yu, Xing
Holloway, Gavan
Santegoets, Kim
Kiefel, Milton J
Blanchard, Helen
Coulson, Barbara S
von Itzstein, Mark
Year published
2009
Metadata
Show full item recordAbstract
We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8*) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides GM1 and GD1a. Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses.We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8*) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides GM1 and GD1a. Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses.
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Journal Title
Nature Chemical Biology
Volume
5
Issue
2
Subject
Medicinal and biomolecular chemistry
Characterisation of biological macromolecules
Biochemistry and cell biology