• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Conference outputs
    • View Item
    • Home
    • Griffith Research Online
    • Conference outputs
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Identification and characterization of the cholesterol 25-hydroxylase gene promoter.

    Author(s)
    Watters, Dianne Josephine
    Nourse, Jamie
    Griffith University Author(s)
    Nourse, Jamie P.
    Watters, Dianne J.
    Year published
    2009
    Metadata
    Show full item record
    Abstract
    25-Hydroxycholesterol (25HC) is the product of the cholesterol 25-hydroxylase (CH25H) gene, and is one of the most potent inhibitors of cholesterol synthesis, through the suppression of SREBP activation. 25HC has also been implicated in neurodegenerative disease and shown to be toxic to primary cultured neuronal cells. Very little is known about the transcriptional regulation of the CH25H gene. Using in silico analysis of the mouse and human promoters, potential binding sites for several transcription factors were identified including p53, CREB and Sp1. The functionality of the putative promoter elements of the mouse promoter ...
    View more >
    25-Hydroxycholesterol (25HC) is the product of the cholesterol 25-hydroxylase (CH25H) gene, and is one of the most potent inhibitors of cholesterol synthesis, through the suppression of SREBP activation. 25HC has also been implicated in neurodegenerative disease and shown to be toxic to primary cultured neuronal cells. Very little is known about the transcriptional regulation of the CH25H gene. Using in silico analysis of the mouse and human promoters, potential binding sites for several transcription factors were identified including p53, CREB and Sp1. The functionality of the putative promoter elements of the mouse promoter was investigated using an in vitro reporter assay with deletion constructs. The results suggest the proximal CREB binding site may be involved in driving expression from the CH25H promoter. While a high proportion TATA-less promoters contain CREB binding sites, these promoters appear to be generally unresponsive to cAMP, suggesting activation of these promoters depends on co-stimulatory signals. In this study we observed co-activation of the CH25H promoter by Sp1 and CREB in a synergistic manner. A negative feedback mechanism involving 25HC was also observed in the in vitro reporter assays and this effect appeared to be mediated by an element within the proximal 250 bp of the promoter. The region containing the p53 consensus site was demonstrated to repress transcription.
    View less >
    Conference Title
    FASEB JOURNAL
    Volume
    23
    Subject
    Biochemistry and cell biology
    Biochemistry and cell biology not elsewhere classified
    Zoology
    Medical physiology
    Publication URI
    http://hdl.handle.net/10072/31549
    Collection
    • Conference outputs

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander