Show simple item record

dc.contributor.authorBrownlie, J.
dc.contributor.authorJohnson, N.
dc.contributor.authorWhyard, S.
dc.date.accessioned2017-05-03T11:46:03Z
dc.date.available2017-05-03T11:46:03Z
dc.date.issued2005
dc.date.modified2010-07-09T07:48:35Z
dc.identifier.issn16174615
dc.identifier.doi10.1007/s00438-005-1110-4
dc.identifier.urihttp://hdl.handle.net/10072/31816
dc.description.abstractThe maT clade of transposons is a group of transposable elements intermediate in sequence and predicted protein structure to mariner and Tc transposons, with a distribution thus far limited to a few invertebrate species. We present evidence, based on searches of publicly available databases, that the nematode Caenorhabditis briggsae has several maT-like transposons, which we have designated as CbmaT elements, dispersed throughout its genome. We also describe two additional transposon sequences that probably share their evolutionary history with the CbmaT transposons. One resembles a fold back variant of a CbmaT element, with long (380-bp) inverted terminal repeats (ITRs) that show a high degree (71%) of identity to CbmaT1. The other, which shares only the 26-bp ITR sequences with one of the CbmaT variants, is present in eight nearly identical copies, but does not have a transposase gene and may therefore be cross mobilised by a CbmaT transposase. Using PCR-based mobility assays,we show that CbmaT1 transposons are capable of excising from the C. briggsae genome. CbmaT1 excised approximately 500 times less frequently than Tcb1 in the reference strain AF16, but both CbmaT1 and Tcb1 excised at extremely high frequencies in the HK105 strain. The HK105 strain also exhibited a high frequency of spontaneous induction of unc-22 mutants, suggesting that it may be a mutator strain of C. briggsae.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer-Verlag GmbH
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom92
dc.relation.ispartofpageto101
dc.relation.ispartofjournalMolecular Genetics and Genomics
dc.relation.ispartofvolume273
dc.rights.retentionY
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode310599
dc.subject.fieldofresearchcode3108
dc.titleThe Caenorhabditis briggsae genome contains active CbmaT1 and Tcb1 transposons
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorBrownlie, Jeremy


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record