Carbonic anhydrase inhibitors. Identification of selective inhibitors of the human mitochondrial isozymes VA and VB over the cytosolic isozymes I and II from a natural product-based phenolic library

Abstract

Mass-directed isolation of the CH2Cl2/MeOH extract from the leaves of Cryptocarya obovata resulted in the purification of a new trypanocidal α-pyrone, 7′,8′-dihydroobolactone (1). The chemical structure of 1 was determined by 1D/2D NMR, MS and CD data analysis. 7′,8′-Dihydroobolactone was shown to inhibit Trypanosoma brucei brucei with an IC50 of 2.8 μM.