Development of conservation strategies for Citrus species of importance to Australia
Author(s)
Hamilton, KN
Ashmore, SE
Drew, RA
Griffith University Author(s)
Year published
2005
Metadata
Show full item recordAbstract
Australian Citrus species have socioeconomic value either as commercially significant fruit crops or as wild relatives of major fruit crops. Long-term conservation of germplasm of these species is important to ensure future access to genetic material for plant improvement projects. An in vitro culture system would allow the mass propagation of these species and assist breeding and conservation programs. In this study, plant regeneration from in vitro nodal cuttings of three Australian Citrus species was achieved. Somatic embryogenesis was also investigated in both cultivated varieties of local importance and native citrus ...
View more >Australian Citrus species have socioeconomic value either as commercially significant fruit crops or as wild relatives of major fruit crops. Long-term conservation of germplasm of these species is important to ensure future access to genetic material for plant improvement projects. An in vitro culture system would allow the mass propagation of these species and assist breeding and conservation programs. In this study, plant regeneration from in vitro nodal cuttings of three Australian Citrus species was achieved. Somatic embryogenesis was also investigated in both cultivated varieties of local importance and native citrus varieties using ovules and seeds. The polyembryonic commercial cultivars (Murcott and Orlando) produced prolific embryogenic callus and somatic embryos that regenerated into plants. C. inodora the only Australian species to form somatic embryos. This embryogenic protocol provided material for a preliminary trial of cryopreservation in C. inodora.
View less >
View more >Australian Citrus species have socioeconomic value either as commercially significant fruit crops or as wild relatives of major fruit crops. Long-term conservation of germplasm of these species is important to ensure future access to genetic material for plant improvement projects. An in vitro culture system would allow the mass propagation of these species and assist breeding and conservation programs. In this study, plant regeneration from in vitro nodal cuttings of three Australian Citrus species was achieved. Somatic embryogenesis was also investigated in both cultivated varieties of local importance and native citrus varieties using ovules and seeds. The polyembryonic commercial cultivars (Murcott and Orlando) produced prolific embryogenic callus and somatic embryos that regenerated into plants. C. inodora the only Australian species to form somatic embryos. This embryogenic protocol provided material for a preliminary trial of cryopreservation in C. inodora.
View less >
Journal Title
Acta Horticulturae
Volume
694
Publisher URI
Subject
Plant biology
Horticultural production