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dc.contributor.authorM. Zalucki, Yaramahen_US
dc.contributor.authorL. Gittins, Karleeen_US
dc.contributor.authorP. Jennings, Michaelen_US
dc.date.accessioned2017-05-03T15:42:35Z
dc.date.available2017-05-03T15:42:35Z
dc.date.issued2008en_US
dc.date.modified2011-10-18T07:24:54Z
dc.identifier.issn0006291Xen_US
dc.identifier.doi10.1016/j.bbrc.2007.11.093en_AU
dc.identifier.urihttp://hdl.handle.net/10072/33007
dc.description.abstractIn this study we altered the codon usage in the signal sequence of the bla gene, encoding beta-lactamase in Escherichia coli. Changing all of the thirteen non-optimal codons to optimal lowered expression 4-fold as measured by minimum inhibitory concentration (MIC) to the beta-lactam antibiotic ampicillin. The difference in ampicillin resistance was reduced at 28 degrees C compared to expression at 37 degrees C, suggesting that the optimised bla allele is misfolded and degraded by heat-shock regulated proteases. A screen was carried out, designed specifically to identify revertants with changes in codon usage resulting in higher MIC to ampicillin. The nine revertants revealed by this method all had optimal to non-optimal codon changes in the signal sequence. These results, and those of our previous study with maltose binding protein model system, confirm that non-optimal codons are important for expression and export of secretory proteins via both the SecB-dependent and -independent pathways.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom135en_US
dc.relation.ispartofpageto141en_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofvolume366en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchProtein Traffickingen_US
dc.subject.fieldofresearchcode060108en_US
dc.titleSecretory signal sequence non-optimal codons are required for expression and export of beta-lactamaseen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2008
gro.hasfulltextNo Full Text


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