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dc.contributor.authorD. Swagell, Christopher
dc.contributor.authorHenly, D.
dc.contributor.authorPhillip Morris, .
dc.date.accessioned2017-05-03T15:52:27Z
dc.date.available2017-05-03T15:52:27Z
dc.date.issued2007
dc.date.modified2010-08-13T07:24:39Z
dc.identifier.issn0006291X
dc.identifier.doi10.1016/j.bbrc.2007.07.191
dc.identifier.urihttp://hdl.handle.net/10072/33058
dc.description.abstractIt is known that fatty acids (FAs) regulate transcription through a number of FA responsive transcription factors. In order to investigate the effect of FAs on gene regulation in cultured human hepatocytes we examined the effect of palmitate on hepatic glucokinase (GK) promoter activity and expression of transcription factors that regulate GK expression. GK promoter activity was increased in constructs lacking a cAMP response element (CRE), while palmitate incubation decreased GK promoter activity in CRE-negative constructs. Cells exposed to palmitate showed increased levels of PPARa apolipoprotein-AII and -B100 mRNA and decreased levels of SREBP-1c mRNA but there was no effect on LXRa and HNF-4a mRNA. In addition, cDNA microarray analysis of short-term (1.5 h) transcriptional regulation by palmitate, oleate, and eicosapentaenoic acid (EPA) identified that oleate and EPA initiated similar changes in the pattern of hepatic gene regulation, whereas palmitate had little effect.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAcademic Press
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom374
dc.relation.ispartofpageto380
dc.relation.ispartofissue2
dc.relation.ispartofjournalBiochemical and biophysical research communications
dc.relation.ispartofvolume362
dc.rights.retentionY
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry not elsewhere classified
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedical Biochemistry and Metabolomics
dc.subject.fieldofresearchcode030499
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1101
dc.titleRegulation of human hepatocyte gene expression by fatty acids
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2007
gro.hasfulltextNo Full Text
gro.griffith.authorHenly, Debra


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