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dc.contributor.authorFindlow, Jamie
dc.contributor.authorTaylor, Stephen
dc.contributor.authorAase, Audun
dc.contributor.authorHorton, Rachel
dc.contributor.authorHeyderman, Robert
dc.contributor.authorSouthern, Jo
dc.contributor.authorAndrews, Nick J.
dc.contributor.authorBarchha, Rita
dc.contributor.authorHarrison, Ewan
dc.contributor.authorLowe, Ann
dc.contributor.authorBoxer, Emma
dc.contributor.authorHeaton, Charlotte
dc.contributor.authorBalmer, Paul
dc.contributor.authorKaczmarski, Ed
dc.contributor.authorOster, Philipp
dc.contributor.authorGorringe, Andrew
dc.contributor.authorBorrow, Ray
dc.contributor.authorMiller, Elizabeth
dc.date.accessioned2017-09-04T02:22:39Z
dc.date.available2017-09-04T02:22:39Z
dc.date.issued2006
dc.date.modified2010-07-30T07:19:51Z
dc.identifier.issn00199567
dc.identifier.urihttp://hdl.handle.net/10072/33073
dc.description.abstractThe prediction of efficacy of Neisseria meningitidis serogroup B (MenB) vaccines is currently hindered due to the lack of an appropriate correlate of protection. For outer membrane vesicle (OMV) vaccines, immunogenicity has primarily been determined by the serum bactericidal antibody (SBA) assay and OMV enzyme-linked immunosorbent assay (ELISA). However, the opsonophagocytic assay (OPA), surface labeling assay, whole blood assay (WBA), and salivary antibody ELISA have been developed although correlation with protection is presently undetermined. Therefore, the aim of the study was to investigate further the usefulness of, and relationships between, MenB immunologic assays. A phase II trial of the OMV vaccine, MenBvac, with proven efficacy was initiated to compare immunologic assays incorporating the vaccine and six heterologous strains. Correlations were achieved between the SBA assay, OMV ELISA, and OPA using human polymorphonuclear leukocytes and human complement but not between an OPA using HL60 phagocytic cells and baby rabbit complement. Correlations between the surface labeling assay, the SBA assay, and the OMV ELISA were promising, although target strain dependent. Correlations between the salivary antibody ELISA and other assays were poor. Correlations to the WBA were prevented since many samples had results greater than the range of the assay. The study confirmed the immunogenicity and benefit of a third dose of MenBvac against the homologous vaccine strain using a variety of immunologic assays. These results emphasize the need for standardized methodologies that would allow a more robust comparison of assays between laboratories and promote their further evaluation as correlates of protection against MenB disease.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom4557
dc.relation.ispartofpageto4565
dc.relation.ispartofissue8
dc.relation.ispartofjournalInfection and Immunity
dc.relation.ispartofvolume74
dc.rights.retentionY
dc.subject.fieldofresearchImmunology not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode110799
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleComparison and Correlation of Neisseria meningitidis Serogroup B Immunologic Assay Results and Human Antibody Responses following Three Doses of the Norwegian Meningococcal Outer Membrane Vesicle Vaccine MenBvac
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2006 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2006
gro.hasfulltextFull Text
gro.griffith.authorHorton, Rachel E.


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