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dc.contributor.authorM. Zalucki, Yaramah
dc.contributor.authorP. Jennings, Michael
dc.date.accessioned2017-05-03T15:42:35Z
dc.date.available2017-05-03T15:42:35Z
dc.date.issued2007
dc.date.modified2010-07-30T07:19:52Z
dc.identifier.issn0006291X
dc.identifier.urihttp://hdl.handle.net/10072/33074
dc.description.abstractNon-optimal codons are defined by low usage and low abundance of corresponding tRNA, and have an established role in translational pausing to allow the correct folding of proteins. Our previous work reported a striking abundance of non-optimal codons in the signal sequences of secretory proteins exported via the sec-dependent pathway in Escherichia coli. In the current study the signal sequence of maltose-binding protein (MBP) was altered so that non-optimal codons were substituted with the most optimal codon from their synonymous codon family. The expression of MBP from the optimized allele (malE-opt) was significantly less than wild-type malE. Expression of MBP from malE-opt was partially restored in a range of cytoplasmic and periplasmic protease deficient strains, confirming that reduced expression of MBP in malE-opt was due to its preferential degradation by cytoplasmic and periplasmic proteases. These data confirm a novel role for non-optimal codon usage in secretion by slowing the rate of translation across the N-terminal signal sequence to facilitate proper folding of the secreted protein.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom143
dc.relation.ispartofpageto148
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiochemical and Biophysical Research Communications
dc.relation.ispartofvolume355
dc.rights.retentionY
dc.subject.fieldofresearchProtein Trafficking
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedical Biochemistry and Metabolomics
dc.subject.fieldofresearchcode060108
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1101
dc.titleExperimental confirmation of a key role for non-optimal codons in protein export
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2007
gro.hasfulltextNo Full Text
gro.griffith.authorJennings, Michael P.
gro.griffith.authorZalucki, Yaramah


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