• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • A genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence

    Thumbnail
    View/Open
    63158_1.pdf (1.765Mb)
    Author(s)
    Nandi, Tannistha
    Ong, Catherine
    Singh, Arvind Pratap
    Boddey, Justin
    Atkins, Timothy
    Sarkar-Tyson, Mitali
    Essex-Lopresti, Angela E
    Chua, Hui Hoon
    Pearson, Talima
    Kreisberg, Jason F
    Nilsson, Christina
    Ariyaratne, Pramila
    Ronning, Catherine
    Losada, Liliana
    Ruan, Yijun
    Sung, Wing-Kin
    Woods, Donald
    Titball, Richard W
    Beacham, Ifor
    Peak, Ian
    Keim, Paul
    Nierman, William C
    Tan, Patrick
    Griffith University Author(s)
    Beacham, Ifor R.
    Peak, Ian
    Boddey, Justin A.
    Year published
    2010
    Metadata
    Show full item record
    Abstract
    Certain environmental microorganisms can cause severe human infections, even in the absence of an obvious requirement for transition through an animal host for replication (''accidental virulence''). To understand this process, we compared eleven isolate genomes of Burkholderia pseudomallei (Bp), a tropical soil microbe and causative agent of the human and animal disease melioidosis. We found evidence for the existence of several new genes in the Bp reference genome, identifying 282 novel genes supported by at least two independent lines of supporting evidence (mRNA transcripts, database homologs, and presence of ribosomal ...
    View more >
    Certain environmental microorganisms can cause severe human infections, even in the absence of an obvious requirement for transition through an animal host for replication (''accidental virulence''). To understand this process, we compared eleven isolate genomes of Burkholderia pseudomallei (Bp), a tropical soil microbe and causative agent of the human and animal disease melioidosis. We found evidence for the existence of several new genes in the Bp reference genome, identifying 282 novel genes supported by at least two independent lines of supporting evidence (mRNA transcripts, database homologs, and presence of ribosomal binding sites) and 81 novel genes supported by all three lines. Within the Bp core genome, 211 genes exhibited significant levels of positive selection (4.5%), distributed across many cellular pathways including carbohydrate and secondary metabolism. Functional experiments revealed that certain positively selected genes might enhance mammalian virulence by interacting with host cellular pathways or utilizing host nutrients. Evolutionary modifications improving Bp environmental fitness may thus have indirectly facilitated the ability of Bp to colonize and survive in mammalian hosts. These findings improve our understanding of the pathogenesis of melioidosis, and establish Bp as a model system for studying the genetics of accidental virulence.
    View less >
    Journal Title
    PLoS Pathogens
    Volume
    6
    Issue
    4
    DOI
    https://doi.org/10.1371/journal.ppat.1000845
    Copyright Statement
    © 2010 Nandi et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
    Subject
    Microbiology
    Infectious agents
    Immunology
    Medical microbiology
    Publication URI
    http://hdl.handle.net/10072/33161
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander