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dc.contributor.authorNandi, Tannistha
dc.contributor.authorOng, Catherine
dc.contributor.authorSingh, Arvind Pratap
dc.contributor.authorBoddey, Justin
dc.contributor.authorAtkins, Timothy
dc.contributor.authorSarkar-Tyson, Mitali
dc.contributor.authorEssex-Lopresti, Angela E
dc.contributor.authorChua, Hui Hoon
dc.contributor.authorPearson, Talima
dc.contributor.authorKreisberg, Jason F
dc.contributor.authorNilsson, Christina
dc.contributor.authorAriyaratne, Pramila
dc.contributor.authorRonning, Catherine
dc.contributor.authorLosada, Liliana
dc.contributor.authorRuan, Yijun
dc.contributor.authorSung, Wing-Kin
dc.contributor.authorWoods, Donald
dc.contributor.authorTitball, Richard W
dc.contributor.authorBeacham, Ifor
dc.contributor.authorPeak, Ian
dc.contributor.authorKeim, Paul
dc.contributor.authorNierman, William C
dc.contributor.authorTan, Patrick
dc.date.accessioned2017-05-03T12:19:14Z
dc.date.available2017-05-03T12:19:14Z
dc.date.issued2010
dc.date.modified2011-04-19T07:03:07Z
dc.identifier.issn1553-7366
dc.identifier.doi10.1371/journal.ppat.1000845
dc.identifier.urihttp://hdl.handle.net/10072/33161
dc.description.abstractCertain environmental microorganisms can cause severe human infections, even in the absence of an obvious requirement for transition through an animal host for replication (''accidental virulence''). To understand this process, we compared eleven isolate genomes of Burkholderia pseudomallei (Bp), a tropical soil microbe and causative agent of the human and animal disease melioidosis. We found evidence for the existence of several new genes in the Bp reference genome, identifying 282 novel genes supported by at least two independent lines of supporting evidence (mRNA transcripts, database homologs, and presence of ribosomal binding sites) and 81 novel genes supported by all three lines. Within the Bp core genome, 211 genes exhibited significant levels of positive selection (4.5%), distributed across many cellular pathways including carbohydrate and secondary metabolism. Functional experiments revealed that certain positively selected genes might enhance mammalian virulence by interacting with host cellular pathways or utilizing host nutrients. Evolutionary modifications improving Bp environmental fitness may thus have indirectly facilitated the ability of Bp to colonize and survive in mammalian hosts. These findings improve our understanding of the pathogenesis of melioidosis, and establish Bp as a model system for studying the genetics of accidental virulence.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1850979 bytes
dc.format.extent78722 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome1000845-1
dc.relation.ispartofpagetoe1000845-15
dc.relation.ispartofissue4
dc.relation.ispartofjournalPLoS Pathogens
dc.relation.ispartofvolume6
dc.rights.retentionY
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchInfectious agents
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310702
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.titleA genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2010 Nandi et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorBeacham, Ifor R.
gro.griffith.authorPeak, Ian


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