Show simple item record

dc.contributor.authorFaglin, Isabelleen_US
dc.contributor.authorTiralongo, Joeen_US
dc.contributor.authorWilson, Jennyen_US
dc.contributor.authorCollins, Patricken_US
dc.contributor.authorPeak, Ianen_US
dc.date.accessioned2017-04-24T09:17:13Z
dc.date.available2017-04-24T09:17:13Z
dc.date.issued2010en_US
dc.date.modified2012-02-10T02:05:17Z
dc.identifier.issn0006291Xen_US
dc.identifier.doi10.1016/j.bbrc.2010.02.028en_US
dc.identifier.urihttp://hdl.handle.net/10072/33169
dc.description.abstractThe lipooligosaccharide (LOS) of Moraxella catarrhalis is unusual in that it lacks heptose. The sugar linking oligosaccharide to Lipid A is a trisubstituted glucose. A single enzyme, Lgt3, is suggested to trisubstitute this core sugar. The lgt3 gene encodes two distinct domains with high similarity to glucosyltransferases of the GT-A superfamily, thus encoding a bidomain, multifunctional glucosyltransferase. To characterise Lgt3, the gene was amplified from M. catarrhalis, expressed in Escherichia coli, and purified. Analysis of its glycosyltransferase catalytic activity ascertained the pH and temperature optima for Lgt3. The donor specificity and acceptor specificity were examined. This study confirms that Lgt3 is a glucosyltransferase which catalyses addition of glucose to its cognate receptor, a terminal glucose presented as the core region of LOS.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationYen_US
dc.relation.ispartofpagefrom609en_US
dc.relation.ispartofpageto613en_US
dc.relation.ispartofissue4en_US
dc.relation.ispartofjournalBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofvolume393en_US
dc.rights.retentionNen_US
dc.subject.fieldofresearchEnzymesen_US
dc.subject.fieldofresearchcode060107en_US
dc.titleBiochemical analysis of Lgt3, a glycosyltransferase of the bacterium Moraxella catarrhalisen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.date.issued2010
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record