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dc.contributor.authorRuhayel, Rasha A
dc.contributor.authorBerners-Price, Susan J
dc.date.accessioned2018-02-13T03:21:15Z
dc.date.available2018-02-13T03:21:15Z
dc.date.issued2010
dc.date.modified2010-09-29T06:54:12Z
dc.identifier.issn0021-9584
dc.identifier.doi10.1021/ed100271h
dc.identifier.urihttp://hdl.handle.net/10072/33186
dc.description.abstractNuclear magnetic resonance (NMR) spectroscopy is an indispensable tool used for the elucidation and confirmation of the solution structure of molecules that was first reported in 1938 by Isidor Rabi (1). A mere eight years later, its use was adapted for structure elucidation of liquids and solids by Felix Bloch and Edward Mills Purcell (2, 3), who four years later shared a Nobel Prize for their work. In more recent times, NMR spectroscopy has been extended to the study of larger biomolecules and has become a technique of paramount importance. In order to introduce students to this field of study, it is imperative that there be a sound understanding of how this technique works. Veeraraghavan describes a short course that effectively introduces students to the basics of NMR spectroscopy (4). A review of the articles available through this Journal shows examples of NMR studies of biological molecules such as peptides, phospholipids, and cytochrome c (5-8); however, there are no examples that use NMR spectroscopy to investigate DNA structure. The work herein represents a 6-h laboratory workshop with the aim of confirming the 3D structure of a short doublestrand DNA sequence, achieved using 2D 1H NOESY NMR spectroscopy, with the aid of a computer-generated model.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom732
dc.relation.ispartofpageto734
dc.relation.ispartofissue7
dc.relation.ispartofjournalJournal of Chemical Education
dc.relation.ispartofvolume87
dc.relation.urihttp://purl.org/au-research/grants/ARC/DP0662817
dc.relation.grantIDDP0662817
dc.relation.fundersARC
dc.rights.retentionY
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchBioinorganic chemistry
dc.subject.fieldofresearchEducation
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode340201
dc.subject.fieldofresearchcode39
dc.titleConfirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2010 American Chemical Society. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorBerners-Price, Sue J.


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