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dc.contributor.authorAkune, Yukie
dc.contributor.authorLin, Chi-Hung
dc.contributor.authorAbrahams, Jodie L
dc.contributor.authorZhang, Jingyu
dc.contributor.authorPacker, Nicolle H
dc.contributor.authorAoki-Kinoshita, Kiyoko F
dc.contributor.authorCampbell, Matthew P
dc.date.accessioned2017-05-11T01:16:51Z
dc.date.available2017-05-11T01:16:51Z
dc.date.issued2016
dc.identifier.issn0008-6215
dc.identifier.doi10.1016/j.carres.2016.05.012
dc.identifier.urihttp://hdl.handle.net/10072/336509
dc.description.abstractGlycan structures attached to proteins are comprised of diverse monosaccharide sequences and linkages that are produced from precursor nucleotide-sugars by a series of glycosyltransferases. Databases of these structures are an essential resource for the interpretation of analytical data and the development of bioinformatics tools. However, with no template to predict what structures are possible the human glycan structure databases are incomplete and rely heavily on the curation of published, experimentally determined, glycan structure data. In this work, a library of 45 human glycosyltransferases was used to generate a theoretical database of N-glycan structures comprised of 15 or less monosaccharide residues. Enzyme specificities were sourced from major online databases including Kyoto Encyclopedia of Genes and Genomes (KEGG) Glycan, Consortium for Functional Glycomics (CFG), Carbohydrate-Active enZymes (CAZy), GlycoGene DataBase (GGDB) and BRENDA. Based on the known activities, more than 1.1 million theoretical structures and 4.7 million synthetic reactions were generated and stored in our database called UniCorn. Furthermore, we analyzed the differences between the predicted glycan structures in UniCorn and those contained in UniCarbKB (www.unicarbkb.org), a database which stores experimentally described glycan structures reported in the literature, and demonstrate that UniCorn can be used to aid in the assignment of ambiguous structures whilst also serving as a discovery database.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPergamon Press
dc.relation.ispartofpagefrom56
dc.relation.ispartofpageto63
dc.relation.ispartofjournalCarbohydrate Research
dc.relation.ispartofvolume431
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchOrganic Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode060199
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0305
dc.subject.fieldofresearchcode0601
dc.titleComprehensive analysis of the N-glycan biosynthetic pathway using bioinformatics to generate UniCorn: A theoretical N-glycan structure database
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorAbrahams, Jodie L.
gro.griffith.authorCampbell, Matthew
gro.griffith.authorPacker, Nicki
gro.griffith.authorLin, Chi-Hung


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