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  • The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets

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    Author(s)
    Struwe, Weston B
    Agravat, Sanjay
    Aoki-Kinoshita, Kiyoko F
    Campbell, Matthew P
    Costello, Catherine E
    Dell, Anne
    Feizi, Ten
    Haslam, Stuart M
    Karlsson, Niclas G
    Khoo, Kay-Hooi
    Kolarich, Daniel
    Liu, Yan
    McBride, Ryan
    Novotny, Milos V
    Packer, Nicolle H
    Paulson, James C
    Rapp, Erdmann
    Ranzinger, Rene
    Rudd, Pauline M
    Smith, David F
    Tiemeyer, Michael
    Wells, Lance
    York, William S
    Zaia, Joseph
    Kettner, Carsten
    Griffith University Author(s)
    Kolarich, Daniel
    Packer, Nicki
    Year published
    2016
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    Abstract
    The minimum information required for a glycomics experiment (MIRAGE) project was established in 2011 to provide guidelines to aid in data reporting from all types of experiments in glycomics research including mass spectrometry (MS), liquid chromatography, glycan arrays, data handling and sample preparation. MIRAGE is a concerted effort of the wider glycomics community that considers the adaptation of reporting guidelines as an important step towards critical evaluation and dissemination of datasets as well as broadening of experimental techniques worldwide. The MIRAGE Commission published reporting guidelines for MS data ...
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    The minimum information required for a glycomics experiment (MIRAGE) project was established in 2011 to provide guidelines to aid in data reporting from all types of experiments in glycomics research including mass spectrometry (MS), liquid chromatography, glycan arrays, data handling and sample preparation. MIRAGE is a concerted effort of the wider glycomics community that considers the adaptation of reporting guidelines as an important step towards critical evaluation and dissemination of datasets as well as broadening of experimental techniques worldwide. The MIRAGE Commission published reporting guidelines for MS data and here we outline guidelines for sample preparation. The sample preparation guidelines include all aspects of sample generation, purification and modification from biological and/or synthetic carbohydrate material. The application of MIRAGE sample preparation guidelines will lead to improved recording of experimental protocols and reporting of understandable and reproducible glycomics datasets.
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    Journal Title
    Glycobiology
    Volume
    26
    Issue
    9
    DOI
    https://doi.org/10.1093/glycob/cww082
    Copyright Statement
    © The Author(s) 2016. Published by Oxford University Press. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
    Subject
    Biological sciences
    Biochemistry and cell biology not elsewhere classified
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/336513
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    • Journal articles

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