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  • MIRAGE: The minimum information required for a glycomics experiment

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    Author(s)
    York, William S
    Agravat, Sanjay
    Aoki-Kinoshita, Kiyoko F
    McBride, Ryan
    Campbell, Matthew P
    Costello, Catherine E
    Dell, Anne
    Feizi, Ten
    Haslam, Stuart M
    Karlsson, Niclas
    Khoo, Kay-Hooi
    Kolarich, Daniel
    Liu, Yan
    Novotny, Milos
    Packer, Nicolle H
    Paulson, James C
    Rapp, Erdmann
    Ranzinger, Rene
    Rudd, Pauline M
    Smith, David F
    Struwe, Weston B
    Tiemeyer, Michael
    Wells, Lance
    Zaia, Joseph
    Kettner, Carsten
    Griffith University Author(s)
    Kolarich, Daniel
    Packer, Nicki
    Year published
    2014
    Metadata
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    Abstract
    The MIRAGE (minimum information required for a glycomics experiment) initiative was founded in Seattle, WA, in November 2011 in order to develop guidelines for reporting the qualitative and quantitative results obtained by diverse types of glycomics analyses, including the conditions and techniques that were applied to prepare the glycans for analysis and generate the primary data along with the tools and parameters that were used to process and annotate this data. These guidelines must address a broad range of issues, as glycomics data are inherently complex and are generated using diverse methods, including mass spectrometry ...
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    The MIRAGE (minimum information required for a glycomics experiment) initiative was founded in Seattle, WA, in November 2011 in order to develop guidelines for reporting the qualitative and quantitative results obtained by diverse types of glycomics analyses, including the conditions and techniques that were applied to prepare the glycans for analysis and generate the primary data along with the tools and parameters that were used to process and annotate this data. These guidelines must address a broad range of issues, as glycomics data are inherently complex and are generated using diverse methods, including mass spectrometry (MS), chromatography, glycan array-binding assays, nuclear magnetic resonance (NMR) and other rapidly developing technologies. The acceptance of these guidelines by scientists conducting research on biological systems in which glycans have a significant role will facilitate the evaluation and reproduction of glycomics experiments and data that is reported in scientific journals and uploaded to glycomics databases. As a first step, MIRAGE guidelines for glycan analysis by MS have been recently published (Kolarich D, Rapp E, Struwe WB, Haslam SM, Zaia J., et al. 2013. The minimum information required for a glycomics experiment (MIRAGE) project – Improving the standards for reporting mass spectrometry-based glycoanalytic data. Mol. Cell Proteomics. 12:991–995), allowing them to be implemented and evaluated in the context of real-world glycobiology research. In this paper, we set out the historical context, organization structure and overarching objectives of the MIRAGE initiative.
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    Journal Title
    Glycobiology
    Volume
    24
    Issue
    5
    DOI
    https://doi.org/10.1093/glycob/cwu018
    Copyright Statement
    © The Author(s) 2014. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
    Subject
    Biological sciences
    Biochemistry and cell biology not elsewhere classified
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/336519
    Collection
    • Journal articles

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