dc.contributor.author | Nguyen-Khuong, Terry | |
dc.contributor.author | Everest-Dass, Arun V | |
dc.contributor.author | Kautto, Liisa | |
dc.contributor.author | Zhao, Zhenjun | |
dc.contributor.author | Willcox, Mark DP | |
dc.contributor.author | Packer, Nicolle H | |
dc.date.accessioned | 2017-05-11T02:57:06Z | |
dc.date.available | 2017-05-11T02:57:06Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0959-6658 | |
dc.identifier.doi | 10.1093/glycob/cwu108 | |
dc.identifier.uri | http://hdl.handle.net/10072/336521 | |
dc.description.abstract | As a secreted fluid, the state of tear glycosylation is particularly important in the role of immunity of
the ocular surface. Tears are a valuable source of non-invasive biomarkers for disease and there are
continued efforts to characterize their components thoroughly. In this study, a small volume of basal
tears (5 μL) was collected from healthy controls, patients with diabetes without retinopathy and patients
with diabetes and retinopathy. The detailed N- and O-linked tear protein glycome was characterized
and the relative abundance of each structure determined. Of the 50 N-linked glycans found,
89% were complex with 50% containing a bisecting N-acetylglucosamine, 65% containing a core fucose
whilst 33% were sialylated. Of the 8 O-linked glycans detected, 3 were of cores 1 and 5 of core
2 type, with a majority of them being sialylated (90%). Additionally, these glycan structures were pro-
filed across the three diabetic disease groups. Whilst the higher abundant structures did not alter
across the three groups, only five low abundance N-linked glycans and 1 O-linked glycan did alter
with the onset of diabetes mellitus and diabetic retinopathy (DR). These results suggest the conservation
of glycan types on basal tear proteins between individuals and point to only small changes in
glycan expression on the proteins in tears with the development of diabetes and DR. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.relation.ispartofpagefrom | 269 | |
dc.relation.ispartofpageto | 283 | |
dc.relation.ispartofissue | 3 | |
dc.relation.ispartofjournal | Glycobiology | |
dc.relation.ispartofvolume | 25 | |
dc.subject.fieldofresearch | Biological sciences | |
dc.subject.fieldofresearch | Biochemistry and cell biology not elsewhere classified | |
dc.subject.fieldofresearch | Biomedical and clinical sciences | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearchcode | 31 | |
dc.subject.fieldofresearchcode | 310199 | |
dc.subject.fieldofresearchcode | 32 | |
dc.subject.fieldofresearchcode | 3101 | |
dc.title | Glycomic characterization of basal tears and changes with diabetes and diabetic retinopathy | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Everest-Dass, Arun | |
gro.griffith.author | Packer, Nicki | |