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dc.contributor.authorNguyen-Khuong, Terry
dc.contributor.authorEverest-Dass, Arun V
dc.contributor.authorKautto, Liisa
dc.contributor.authorZhao, Zhenjun
dc.contributor.authorWillcox, Mark DP
dc.contributor.authorPacker, Nicolle H
dc.date.accessioned2017-05-11T02:57:06Z
dc.date.available2017-05-11T02:57:06Z
dc.date.issued2015
dc.identifier.issn0959-6658
dc.identifier.doi10.1093/glycob/cwu108
dc.identifier.urihttp://hdl.handle.net/10072/336521
dc.description.abstractAs a secreted fluid, the state of tear glycosylation is particularly important in the role of immunity of the ocular surface. Tears are a valuable source of non-invasive biomarkers for disease and there are continued efforts to characterize their components thoroughly. In this study, a small volume of basal tears (5 μL) was collected from healthy controls, patients with diabetes without retinopathy and patients with diabetes and retinopathy. The detailed N- and O-linked tear protein glycome was characterized and the relative abundance of each structure determined. Of the 50 N-linked glycans found, 89% were complex with 50% containing a bisecting N-acetylglucosamine, 65% containing a core fucose whilst 33% were sialylated. Of the 8 O-linked glycans detected, 3 were of cores 1 and 5 of core 2 type, with a majority of them being sialylated (90%). Additionally, these glycan structures were pro- filed across the three diabetic disease groups. Whilst the higher abundant structures did not alter across the three groups, only five low abundance N-linked glycans and 1 O-linked glycan did alter with the onset of diabetes mellitus and diabetic retinopathy (DR). These results suggest the conservation of glycan types on basal tear proteins between individuals and point to only small changes in glycan expression on the proteins in tears with the development of diabetes and DR.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofpagefrom269
dc.relation.ispartofpageto283
dc.relation.ispartofissue3
dc.relation.ispartofjournalGlycobiology
dc.relation.ispartofvolume25
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiochemistry and cell biology not elsewhere classified
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310199
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode3101
dc.titleGlycomic characterization of basal tears and changes with diabetes and diabetic retinopathy
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorEverest-Dass, Arun
gro.griffith.authorPacker, Nicki


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