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dc.contributor.authorAnugraham, Merrina
dc.contributor.authorJacob, Francis
dc.contributor.authorNixdorf, Sheri
dc.contributor.authorEverest-Dass, Arun Vijay
dc.contributor.authorHeinzelmann-Schwarz, Viola
dc.contributor.authorPacker, Nicolle H
dc.date.accessioned2017-05-11T03:51:33Z
dc.date.available2017-05-11T03:51:33Z
dc.date.issued2014
dc.identifier.issn1535-9476
dc.identifier.doi10.1074/mcp.M113.037085
dc.identifier.urihttp://hdl.handle.net/10072/336536
dc.description.abstractEpithelial ovarian cancer is the fifth most common cause of cancer in women worldwide bearing the highest mortality rate among all gynecological cancers. Cell membrane glycans mediate various cellular processes such as cell signaling and become altered during carcinogenesis. The extent to which glycosylation changes are influenced by aberrant regulation of gene expression is nearly unknown for ovarian cancer and remains crucial in understanding the development and progression of this disease. To address this effect, we analyzed the membrane glycosylation of non-cancerous ovarian surface epithelial (HOSE 6.3 and HOSE 17.1) and serous ovarian cancer cell lines (SKOV 3, IGROV1, A2780, and OVCAR 3), the most common histotype among epithelial ovarian cancers. N-glycans were released from membrane glycoproteins by PNGase F and analyzed using nano-liquid chromatography on porous graphitized carbon and negative-ion electrospray ionization mass spectrometry (ESI-MS). Glycan structures were characterized based on their molecular masses and tandem MS fragmentation patterns. We identified characteristic glycan features that were unique to the ovarian cancer membrane proteins, namely the “bisecting N-acetyl-glucosamine” type N-glycans, increased levels of α 2–6 sialylated N-glycans and “N,N′-diacetyl-lactosamine” type N-glycans. These N-glycan changes were verified by examining gene transcript levels of the enzymes specific for their synthesis (MGAT3, ST6GAL1, and B4GALNT3) using qRT-PCR. We further evaluated the potential epigenetic influence on MGAT3 expression by treating the cell lines with 5-azacytidine, a DNA methylation inhibitor. For the first time, we provide evidence that MGAT3 expression may be epigenetically regulated by DNA hypomethylation, leading to the synthesis of the unique “bisecting GlcNAc” type N-glycans on the membrane proteins of ovarian cancer cells. Linking the observation of specific N-glycan substructures and their complex association with epigenetic programming of their associated synthetic enzymes in ovarian cancer could potentially be used for the development of novel anti-glycan drug targets and clinical diagnostic tools.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.relation.ispartofpagefrom2213
dc.relation.ispartofpageto2232
dc.relation.ispartofissue9
dc.relation.ispartofjournalMolecular and Cellular Proteomics
dc.relation.ispartofvolume13
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titleSpecific glycosylation of membrane proteins in epithelial ovarian cancer cell lines: Glycan structures reflect gene expression and DNA methylation status
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyrightThis research was originally published in Molecular & Cellular Proteomics (MCP). Anugraham et al, Specific glycosylation of membrane proteins in epithelial ovarian cancer cell lines: Glycan structures reflect gene expression and DNA methylation status, Molecular & Cellular Proteomics (MCP), 2014; 13(9): 2213-2232. Copyright the American Society for Biochemistry and Molecular Biology. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitve version.
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gro.griffith.authorEverest-Dass, Arun
gro.griffith.authorPacker, Nicki


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