Show simple item record

dc.contributor.authorLeymarie, Nancy
dc.contributor.authorGriffin, Paula J
dc.contributor.authorJonscher, Karen
dc.contributor.authorKolarich, Daniel
dc.contributor.authorOrlando, Ron
dc.contributor.authorMcComb, Mark
dc.contributor.authorZaia, Joseph
dc.contributor.authorAguilan, Jennifer
dc.contributor.authorAlley, William R
dc.contributor.authorAltmann, Friederich
dc.contributor.authorBall, Lauren E
dc.contributor.authorBasumallick, Lipika
dc.contributor.authorBazemore-Walker, Carthene R
dc.contributor.authorBehnken, Henning
dc.contributor.authorBlank, Michael A
dc.contributor.authorBrown, Kristy J
dc.contributor.authorBunz, Svenja-Catharina
dc.contributor.authorCairo, Christopher W
dc.contributor.authorCipollo, John F
dc.contributor.authorDaneshfar, Rambod
dc.contributor.authorDesaire, Heather
dc.contributor.authorDrake, Richard R
dc.contributor.authorGo, Eden P
dc.contributor.authorGoldman, Radoslav
dc.contributor.authorGruber, Clemens
dc.contributor.authorHalim, Adnan
dc.contributor.authorHathout, Yetrib
dc.contributor.authorHensbergen, Paul J
dc.contributor.authorHorn, David M
dc.contributor.authorHurum, Deanna
dc.contributor.authorJabs, Wolfgang
dc.contributor.authorLarson, Goran
dc.contributor.authorLy, Mellisa
dc.contributor.authorMann, Benjamin F
dc.contributor.authorMarx, Kristina
dc.contributor.authorMechref, Yehia
dc.contributor.authorMeyer, Bernd
dc.contributor.authorMoeginger, Uwe
dc.contributor.authorNeusuess, Christian
dc.contributor.authorNilsson, Jonas
dc.contributor.authorNovotny, Milos V
dc.contributor.authorNyalwidhe, Julius O
dc.contributor.authorPacker, Nicolle H
dc.contributor.authorPompach, Petr
dc.contributor.authorReiz, Bela
dc.contributor.authorResemann, Anja
dc.contributor.authorRohrer, Jeffrey S
dc.contributor.authorRuthenbeck, Alexandra
dc.contributor.authorSanda, Miloslav
dc.contributor.authorSchulz, Jan Mirco
dc.contributor.authorSchweiger-Hufnagel, Ulrike
dc.contributor.authorSihlbom, Carina
dc.contributor.authorSong, Ehwang
dc.contributor.authorStaples, Gregory O
dc.contributor.authorSuckau, Detlev
dc.contributor.authorTang, Haixu
dc.contributor.authorThaysen-Andersen, Morten
dc.contributor.authorViner, Rosa I
dc.contributor.authorAn, Yanming
dc.contributor.authorValmuv, Leena
dc.contributor.authorWada, Yoshinao
dc.contributor.authorWatson, Megan
dc.contributor.authorWindwarder, Markus
dc.contributor.authorWhittal, Randy
dc.contributor.authorWuhrer, Manfred
dc.contributor.authorZhu, Yiying
dc.contributor.authorZou, Chunxia
dc.date.accessioned2017-05-11T03:59:08Z
dc.date.available2017-05-11T03:59:08Z
dc.date.issued2013
dc.identifier.issn1535-9476
dc.identifier.doi10.1074/mcp.M113.030643
dc.identifier.urihttp://hdl.handle.net/10072/336542
dc.description.abstractOne of the principal goals of glycoprotein research is to correlate glycan structure and function. Such correlation is necessary in order for one to understand the mechanisms whereby glycoprotein structure elaborates the functions of myriad proteins. The accurate comparison of glycoforms and quantification of glycosites are essential steps in this direction. Mass spectrometry has emerged as a powerful analytical technique in the field of glycoprotein characterization. Its sensitivity, high dynamic range, and mass accuracy provide both quantitative and sequence/structural information. As part of the 2012 ABRF Glycoprotein Research Group study, we explored the use of mass spectrometry and ancillary methodologies to characterize the glycoforms of two sources of human prostate specific antigen (PSA). PSA is used as a tumor marker for prostate cancer, with increasing blood levels used to distinguish between normal and cancer states. The glycans on PSA are believed to be biantennary N-linked, and it has been observed that prostate cancer tissues and cell lines contain more antennae than their benign counterparts. Thus, the ability to quantify differences in glycosylation associated with cancer has the potential to positively impact the use of PSA as a biomarker. We studied standard peptide-based proteomics/glycomics methodologies, including LC-MS/MS for peptide/glycopeptide sequencing and label-free approaches for differential quantification. We performed an interlaboratory study to determine the ability of different laboratories to correctly characterize the differences between glycoforms from two different sources using mass spectrometry methods. We used clustering analysis and ancillary statistical data treatment on the data sets submitted by participating laboratories to obtain a consensus of the glycoforms and abundances. The results demonstrate the relative strengths and weaknesses of top-down glycoproteomics, bottom-up glycoproteomics, and glycomics methods.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.relation.ispartofpagefrom2935
dc.relation.ispartofpageto2951
dc.relation.ispartofissue10
dc.relation.ispartofjournalMolecular and Cellular Proteomics
dc.relation.ispartofvolume12
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titleInterlaboratory study on differential analysis of protein glycosylation by mass spectrometry: The ABRF glycoprotein research multi-institutional study 2012
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyrightThis research was originally published in Molecular & Cellular Proteomics (MCP). Leymarie et al, Interlaboratory study on differential analysis of protein glycosylation by mass spectrometry: The ABRF glycoprotein research multi-institutional study 2012, Molecular & Cellular Proteomics (MCP), 2013; 12(10): 2935-2951. Copyright the American Society for Biochemistry and Molecular Biology. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitve version.
gro.hasfulltextFull Text
gro.griffith.authorKolarich, Daniel
gro.griffith.authorPacker, Nicki


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record