Microwave assisted prepared Interpenetrating hydrogels from guar-gum: chitosan IPN and guar gum hydrogels as novel functional materials: bonding, antioxidant and bioactivity
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The interpenetrating polymeric network (IPN) has emerged as one of the most useful novel biomaterial, which is entanglement of polymer networks of structurally and functionally different cross-linked molecular building blocks. The development of IPN is captivating because they provide free volume space for the easy encapsulation of drugs in the three-dimensional network structure, formulated by cross-linking of several distinct polymer network. Chitosan and guar gum are a natural, biodegradable, nontoxic, mucoadhesive, and biocompatible polymer, have found diverse pharmaceutical applications including functional biomaterials. Cytotoxicity of common dental resin components are well documented and is prone to cause the undesired biological responses such as oxidative damage of dental and related tissue as well as suppressing odontogenic differentiation of dental pulp cells. As antioxidants were found to protect cells from cytotoxicity of resin monomers in previous studies, we investigated the effects of common antioxidants, such as β-carotene, resveratrol, and propolis on anti-differentiation activity of bonding agents without compromising bond strength. Methacrylate monomers used in dentistry have shown to induce DNA double strand breaks (DSBs), a severe type of DNA damage. The formation of classical products of oxidative DNA damage, were investigated using the well established and reliable in vitro model, developed earlier by us which relies on correlation of BSA solubility and the amount of free radicals generated at the reaction site using UV-detection method. In this work, we hope to develop and evaluated novel functional biomaterials suitable for ameliorating dentin bonding system as well as asses in vitro the free radical protective properties of the novel materials by utilizing earlier developed model protein (BSA) probe was utilized to evaluate the chitosan-based carriers. Morphological behaviors, release behaviors (physiological pH and in acidic conditions) and stability guar gum: chitosan systems have been investigated. We considered worthwhile to study the antioxidant properties of β-carotene, resveratrol, and propolis as examples of the potential antioxidant additiives.Conclusion: Antioxidant containing chitosan hydrogels may reduce detrimental effects induced by common composite restorative agent in vitro and introducing additional therapeutic health benefits
Biointerface Research in Applied Chemistry
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Dental Materials and Equipment