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dc.contributor.authorE. Horton, Rachelen_US
dc.contributor.authorKaefer, Nadineen_US
dc.contributor.authorSongok, Elijahen_US
dc.contributor.authorB. Guijon, Fernandoen_US
dc.contributor.authorKettaf, Nadiaen_US
dc.contributor.authorBoucher, Genevieveen_US
dc.contributor.authorSekaly, Rafick-Pierreen_US
dc.contributor.authorBlake Ball, T.en_US
dc.contributor.authorA. Plummer, Franken_US
dc.date.accessioned2017-05-03T15:42:33Z
dc.date.available2017-05-03T15:42:33Z
dc.date.issued2009en_US
dc.date.modified2010-10-27T08:29:54Z
dc.identifier.issn19326203en_US
dc.identifier.doi10.1371/journal.pone.0008293en_AU
dc.identifier.urihttp://hdl.handle.net/10072/33895
dc.description.abstractStudies of the immunological environment in the female genital tract (FGT) are critical for the development of vaccines or microbicides to halt the spread of sexually transmitted infections. Challenges arise due to the difficulties of sampling from this site, and the majority of studies have been conducted utilising peripheral blood mononuclear cells. Identifying functional differences between immune cells of the FGT and peripheral blood would aid in our understanding of mucosal immunology. We compared the gene expression profile of mononuclear cells at these two sites. Messenger RNA expression analysis was performed using gene expression arrays on matched cervical mononuclear cells and peripheral blood mononuclear cells. Further cellular phenotyping was done by 10 colour flow cytometry. Of the 22,185 genes expressed by these samples, 5345 genes were significantly differentially expressed between the cell populations. Most differences can be explained by significantly lower levels of T and B cells and higher levels of macrophages and dendritic cells in the FGT compared with peripheral blood. Several immunologically relevant pathways such as apoptosis and innate immune signalling, and a variety of cytokines and cytokine receptors were differentially expressed. This study highlights the importance of the unique immunological environment of the FGT and identifies important differences between systemic and mucosal immune compartments.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.format.extent1351503 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherPublic Library of Scienceen_US
dc.publisher.placeSan Francisco, CAen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrome8293-1en_US
dc.relation.ispartofpagetoe8293-9en_US
dc.relation.ispartofissue12en_US
dc.relation.ispartofjournalPLoS Oneen_US
dc.relation.ispartofvolume4en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchImmunology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110799en_US
dc.titleA Comparative Analysis of Gene Expression Patterns and Cell Phenotypes between Cervical and Peripheral Blood Mononuclear Cellsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dcterms.licensehttp://www.plos.org/journals/license.htmlen_US
gro.rights.copyrightCopyright 2009 Horton et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)en_AU
gro.date.issued2009
gro.hasfulltextFull Text


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