Micro in-focal cell stretching platform with parallel programmable control
This paper reports a micro-scale programmable cell stretching platform. The platform keeps the cell monolayer in the microscopic focal plane throughout the stretching process allowing real-time imaging of the cells with magnifications up to 40×, and post-stretching optical analysis with magnification up to 100×. The device consists of highly parallelized stretching array of 15 units with reconfigurable strains. Each unit was an assembly of three elastomeric layers and a bottom glass cover-slip. In the middle of the assembly, an 8 μm thick membrane is used as the stretching substrate for the cells. A programmable pneumatic control system provides actuation. The stretching of the membrane is measured in situ using µPIV technique. The results show that a uniform strain of above 60% can be achieved. Preliminary cell stretching experiments in this device with 5 different cell lines were conducted. Both the acute cell deformation and chronic morphological changes in response to the cyclic strain have confirmed the functionality of this platform.
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)
Medical Biotechnology not elsewhere classified