Micro in-focal cell stretching platform with parallel programmable control
Author(s)
Huang, Y
Nguyen, NT
Griffith University Author(s)
Year published
2012
Metadata
Show full item recordAbstract
This paper reports a micro-scale programmable cell stretching platform. The platform keeps the cell monolayer
in the microscopic focal plane throughout the stretching process allowing real-time imaging of the cells with
magnifications up to 40×, and post-stretching optical analysis with magnification up to 100×. The device consists of
highly parallelized stretching array of 15 units with reconfigurable strains. Each unit was an assembly of three
elastomeric layers and a bottom glass cover-slip. In the middle of the assembly, an 8 μm thick membrane is used as
the stretching substrate for the cells. A programmable pneumatic ...
View more >This paper reports a micro-scale programmable cell stretching platform. The platform keeps the cell monolayer in the microscopic focal plane throughout the stretching process allowing real-time imaging of the cells with magnifications up to 40×, and post-stretching optical analysis with magnification up to 100×. The device consists of highly parallelized stretching array of 15 units with reconfigurable strains. Each unit was an assembly of three elastomeric layers and a bottom glass cover-slip. In the middle of the assembly, an 8 μm thick membrane is used as the stretching substrate for the cells. A programmable pneumatic control system provides actuation. The stretching of the membrane is measured in situ using µPIV technique. The results show that a uniform strain of above 60% can be achieved. Preliminary cell stretching experiments in this device with 5 different cell lines were conducted. Both the acute cell deformation and chronic morphological changes in response to the cyclic strain have confirmed the functionality of this platform.
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View more >This paper reports a micro-scale programmable cell stretching platform. The platform keeps the cell monolayer in the microscopic focal plane throughout the stretching process allowing real-time imaging of the cells with magnifications up to 40×, and post-stretching optical analysis with magnification up to 100×. The device consists of highly parallelized stretching array of 15 units with reconfigurable strains. Each unit was an assembly of three elastomeric layers and a bottom glass cover-slip. In the middle of the assembly, an 8 μm thick membrane is used as the stretching substrate for the cells. A programmable pneumatic control system provides actuation. The stretching of the membrane is measured in situ using µPIV technique. The results show that a uniform strain of above 60% can be achieved. Preliminary cell stretching experiments in this device with 5 different cell lines were conducted. Both the acute cell deformation and chronic morphological changes in response to the cyclic strain have confirmed the functionality of this platform.
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Conference Title
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher URI
Subject
Medical biotechnology not elsewhere classified