dc.contributor.author | Engelbrecht, AM | |
dc.contributor.author | Odendaal, L | |
dc.contributor.author | Du Toit, EF | |
dc.contributor.author | Kupai, K | |
dc.contributor.author | Csont, T | |
dc.contributor.author | Ferdinandy, P | |
dc.contributor.author | Van Rooyen, J | |
dc.date.accessioned | 2017-05-03T15:31:50Z | |
dc.date.available | 2017-05-03T15:31:50Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1476-511X | |
dc.identifier.doi | 10.1186/1476-511X-8-18 | |
dc.identifier.uri | http://hdl.handle.net/10072/33956 | |
dc.description.abstract | We have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia/reperfusion-induced injury. Unfortunately, the cellular and molecular mechanisms responsible for this phenomenon are still poorly understood and no knowledge exists regarding the effects of RPO supplementation on the phosphoinositide 3-kinase (PI3-K) signaling pathway and apoptosis during ischaemia/reperfusion injury. Therefore, the aims of the present study were three fold: (i) to establish the effect of RPO on the functional recovery of the heart after ischaemia/reperfuion injury; (ii) to determine the effect of the PI3-K pathway in RPO-induced protection with the aid of an inhibitor (wortmannin); and (iii) to evaluate apoptosis in our model. Wistar rats were fed a standard rat chow control diet or a control diet plus 7 g RPO/kg for six weeks. Hearts were excised and mounted on a Langendorff perfusion apparatus. Mechanical function was measured after a 25 min period of total global ischaemia followed by 30 minutes of reperfusion. Hearts subjected to the same conditions were freeze-clamped for biochemical analysis at 10 min during reperfusion to determine the involvement of the PI3-Kinase signaling pathway and apoptosis in our model. Dietary RPO supplementation significantly increased % rate pressure product recovery during reperfusion (71.0 ᠶ.3% in control vs 92.36 ᠴ.489% in RPO; p < 0.05). The % rate pressure product recovery was significantly reduced when wortmannin was added during perfusion (92.36 ᠴ.489% in the RPO group vs 75.21 ᠵ.26% in RPO + Wm). RPO + Wm also significantly attenuated PI3-K induction compared with the RPO group (59.2 ᠲ.8 pixels in RPO vs 37.9 ᠳ.4 pixels in RPO + Wm). We have also demonstrated that PI3-K inhibition induced PARP cleavage (marker of apoptosis) in the hearts during ischaemia/reperfusion injury and that RPO supplementation counteracted this effect. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 479827 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | BioMed Central | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 18 | |
dc.relation.ispartofpageto | 24 | |
dc.relation.ispartofjournal | Lipids in Health and Disease | |
dc.relation.ispartofvolume | 8 | |
dc.rights.retention | N | |
dc.subject.fieldofresearch | Other information and computing sciences | |
dc.subject.fieldofresearch | Medical biochemistry and metabolomics | |
dc.subject.fieldofresearch | Cardiology (incl. cardiovascular diseases) | |
dc.subject.fieldofresearch | Nutrition and dietetics | |
dc.subject.fieldofresearch | Medical physiology not elsewhere classified | |
dc.subject.fieldofresearchcode | 4699 | |
dc.subject.fieldofresearchcode | 3205 | |
dc.subject.fieldofresearchcode | 320101 | |
dc.subject.fieldofresearchcode | 3210 | |
dc.subject.fieldofresearchcode | 320899 | |
dc.title | The effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart: the involvement of the PI3-Kinase signaling pathway. | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | http://creativecommons.org/licenses/by/2.0 | |
gro.rights.copyright | © 2009 Engelbrecht et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
gro.date.issued | 2015-05-31T22:20:48Z | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Du Toit, Eugene | |