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dc.contributor.authorEngelbrecht, AM
dc.contributor.authorOdendaal, L
dc.contributor.authorDu Toit, EF
dc.contributor.authorKupai, K
dc.contributor.authorCsont, T
dc.contributor.authorFerdinandy, P
dc.contributor.authorVan Rooyen, J
dc.date.accessioned2017-05-03T15:31:50Z
dc.date.available2017-05-03T15:31:50Z
dc.date.issued2009
dc.identifier.issn1476-511X
dc.identifier.doi10.1186/1476-511X-8-18
dc.identifier.urihttp://hdl.handle.net/10072/33956
dc.description.abstractWe have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia/reperfusion-induced injury. Unfortunately, the cellular and molecular mechanisms responsible for this phenomenon are still poorly understood and no knowledge exists regarding the effects of RPO supplementation on the phosphoinositide 3-kinase (PI3-K) signaling pathway and apoptosis during ischaemia/reperfusion injury. Therefore, the aims of the present study were three fold: (i) to establish the effect of RPO on the functional recovery of the heart after ischaemia/reperfuion injury; (ii) to determine the effect of the PI3-K pathway in RPO-induced protection with the aid of an inhibitor (wortmannin); and (iii) to evaluate apoptosis in our model. Wistar rats were fed a standard rat chow control diet or a control diet plus 7 g RPO/kg for six weeks. Hearts were excised and mounted on a Langendorff perfusion apparatus. Mechanical function was measured after a 25 min period of total global ischaemia followed by 30 minutes of reperfusion. Hearts subjected to the same conditions were freeze-clamped for biochemical analysis at 10 min during reperfusion to determine the involvement of the PI3-Kinase signaling pathway and apoptosis in our model. Dietary RPO supplementation significantly increased % rate pressure product recovery during reperfusion (71.0 ᠶ.3% in control vs 92.36 ᠴ.489% in RPO; p < 0.05). The % rate pressure product recovery was significantly reduced when wortmannin was added during perfusion (92.36 ᠴ.489% in the RPO group vs 75.21 ᠵ.26% in RPO + Wm). RPO + Wm also significantly attenuated PI3-K induction compared with the RPO group (59.2 ᠲ.8 pixels in RPO vs 37.9 ᠳ.4 pixels in RPO + Wm). We have also demonstrated that PI3-K inhibition induced PARP cleavage (marker of apoptosis) in the hearts during ischaemia/reperfusion injury and that RPO supplementation counteracted this effect.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent479827 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom18
dc.relation.ispartofpageto24
dc.relation.ispartofjournalLipids in Health and Disease
dc.relation.ispartofvolume8
dc.rights.retentionN
dc.subject.fieldofresearchOther information and computing sciences
dc.subject.fieldofresearchMedical biochemistry and metabolomics
dc.subject.fieldofresearchCardiology (incl. cardiovascular diseases)
dc.subject.fieldofresearchNutrition and dietetics
dc.subject.fieldofresearchMedical physiology not elsewhere classified
dc.subject.fieldofresearchcode4699
dc.subject.fieldofresearchcode3205
dc.subject.fieldofresearchcode320101
dc.subject.fieldofresearchcode3210
dc.subject.fieldofresearchcode320899
dc.titleThe effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart: the involvement of the PI3-Kinase signaling pathway.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
gro.rights.copyright© 2009 Engelbrecht et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2015-05-31T22:20:48Z
gro.hasfulltextFull Text
gro.griffith.authorDu Toit, Eugene


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