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dc.contributor.authorEngelbrecht, Anna-Marten_US
dc.contributor.authorOdendaal, Louiseen_US
dc.contributor.authorDu Toit, Eugeneen_US
dc.contributor.authorKupai, Kristinaen_US
dc.contributor.authorCsont, Tamásen_US
dc.contributor.authorFerdinandy, Peteren_US
dc.contributor.authorvan Rooyen, Jacquesen_US
dc.date.accessioned2017-04-24T13:06:14Z
dc.date.available2017-04-24T13:06:14Z
dc.date.issued2009en_US
dc.identifier.issn1476511Xen_US
dc.identifier.doi10.1186/1476-511X-8-18en_US
dc.identifier.urihttp://hdl.handle.net/10072/33956
dc.description.abstractWe have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia/reperfusion-induced injury. Unfortunately, the cellular and molecular mechanisms responsible for this phenomenon are still poorly understood and no knowledge exists regarding the effects of RPO supplementation on the phosphoinositide 3-kinase (PI3-K) signaling pathway and apoptosis during ischaemia/reperfusion injury. Therefore, the aims of the present study were three fold: (i) to establish the effect of RPO on the functional recovery of the heart after ischaemia/reperfuion injury; (ii) to determine the effect of the PI3-K pathway in RPO-induced protection with the aid of an inhibitor (wortmannin); and (iii) to evaluate apoptosis in our model. Wistar rats were fed a standard rat chow control diet or a control diet plus 7 g RPO/kg for six weeks. Hearts were excised and mounted on a Langendorff perfusion apparatus. Mechanical function was measured after a 25 min period of total global ischaemia followed by 30 minutes of reperfusion. Hearts subjected to the same conditions were freeze-clamped for biochemical analysis at 10 min during reperfusion to determine the involvement of the PI3-Kinase signaling pathway and apoptosis in our model. Dietary RPO supplementation significantly increased % rate pressure product recovery during reperfusion (71.0 ᠶ.3% in control vs 92.36 ᠴ.489% in RPO; p < 0.05). The % rate pressure product recovery was significantly reduced when wortmannin was added during perfusion (92.36 ᠴ.489% in the RPO group vs 75.21 ᠵ.26% in RPO + Wm). RPO + Wm also significantly attenuated PI3-K induction compared with the RPO group (59.2 ᠲ.8 pixels in RPO vs 37.9 ᠳ.4 pixels in RPO + Wm). We have also demonstrated that PI3-K inhibition induced PARP cleavage (marker of apoptosis) in the hearts during ischaemia/reperfusion injury and that RPO supplementation counteracted this effect.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent479827 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherBioMed Centralen_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom18en_US
dc.relation.ispartofpageto24en_US
dc.relation.ispartofjournalLipids in Health and Diseaseen_US
dc.relation.ispartofvolume8en_US
dc.rights.retentionNen_US
dc.subject.fieldofresearchCardiology (incl. Cardiovascular Diseases)en_US
dc.subject.fieldofresearchMedical Physiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110201en_US
dc.subject.fieldofresearchcode111699en_US
dc.titleThe effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart: the involvement of the PI3-Kinase signaling pathway.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2009 Author 1 surname] et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
gro.date.issued2015-05-31T22:20:48Z
gro.hasfulltextFull Text


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