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  • The minimum information required for a glycomics experiment (MIRAGE) project: Improving the standards for reporting mass-spectrometry-based glycoanalytic data

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    KolarichPUB2526.pdf (772.9Kb)
    Author(s)
    Kolarich, Daniel
    Rapp, Erdmann
    Struwe, Weston B
    Haslam, Stuart M
    Zaia, Joseph
    McBride, Ryan
    Agravat, Sanjay
    Campbell, Matthew P
    Kato, Masaki
    Ranzinger, Rene
    Kettner, Carsten
    York, William S
    Griffith University Author(s)
    Kolarich, Daniel
    Campbell, Matthew
    Year published
    2013
    Metadata
    Show full item record
    Abstract
    The MIRAGE guidelines are being developed in response to a critical need in the glycobiology community to clarify glycoanalytic results so that they are more readily evaluated (in terms of their scope and depth) and to facilitate the reproduction of important results in the laboratory. The molecular and biological complexity of the glycosylation process makes thorough reporting of the results of a glycomics experiment a highly challenging endeavor. The resulting data specify the identity and quantity of complex structures, the precise molecular features of which are sometimes inferred using prior knowledge, such as familiarity ...
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    The MIRAGE guidelines are being developed in response to a critical need in the glycobiology community to clarify glycoanalytic results so that they are more readily evaluated (in terms of their scope and depth) and to facilitate the reproduction of important results in the laboratory. The molecular and biological complexity of the glycosylation process makes thorough reporting of the results of a glycomics experiment a highly challenging endeavor. The resulting data specify the identity and quantity of complex structures, the precise molecular features of which are sometimes inferred using prior knowledge, such as familiarity with a particular biosynthetic mechanism. Specifying the exact methods and assumptions that were used to assign and quantify reported structures allows the interested scientist to appreciate the scope and depth of the analysis. Mass spectrometry (MS) is the most widely used tool for glycomics experiments. The interpretation and reproducibility of MS-based glycomics data depend on comprehensive meta-data describing the instrumentation, instrument setup, and data acquisition protocols. The MIRAGE guidelines for MS-based glycomics have been designed to facilitate the collection and sharing of this critical information in order to assist the glycoanalyst in generating data sets with maximum information content and biological relevance.
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    Journal Title
    Molecular and Cellular Proteomics
    Volume
    12
    Issue
    4
    DOI
    https://doi.org/10.1074/mcp.O112.026492
    Copyright Statement
    This research was originally published in Molecular & Cellular Proteomics (MCP). Kolarich et al, The minimum information required for a glycomics experiment (MIRAGE) project: Improving the standards for reporting mass-spectrometry-based glycoanalytic data, Molecular & Cellular Proteomics (MCP)  2013; 12: 991-995. Copyright the American Society for Biochemistry and Molecular Biology. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitve version.
    Subject
    Analytical Chemistry not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/339715
    Collection
    • Journal articles

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