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  • Discovery and structural characterization of fucosylated oligomannosidic N-glycans in mushrooms

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    Author(s)
    Grass, Josephine
    Pabst, Martin
    Kolarich, Daniel
    Poeltl, Gerald
    Leonard, Renaud
    Brecker, Lothar
    Altmann, Friedrich
    Griffith University Author(s)
    Kolarich, Daniel
    Year published
    2011
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    Abstract
    L-Fucose is a common constituent of Asn-linked glycans in vertebrates, invertebrates, and plants, but in fungal glycoproteins, fucose has not been found so far. However, by mass spectrometry we detected N-glycans and O-glycans containing one to six deoxyhexose residues in fruit bodies of several basidiomycetes. The N-glycans of chanterelles (Cantharellus cibarius) contained a deoxyhexose chromatographically identical to fucose and sensitive to α-L-fucosidase. Analysis of individual glycan species by tandem MS, glycosidase digestion, and finally 1H NMR revealed the presence of L-fucose in α1,6-linkage to an α1,6-mannose of ...
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    L-Fucose is a common constituent of Asn-linked glycans in vertebrates, invertebrates, and plants, but in fungal glycoproteins, fucose has not been found so far. However, by mass spectrometry we detected N-glycans and O-glycans containing one to six deoxyhexose residues in fruit bodies of several basidiomycetes. The N-glycans of chanterelles (Cantharellus cibarius) contained a deoxyhexose chromatographically identical to fucose and sensitive to α-L-fucosidase. Analysis of individual glycan species by tandem MS, glycosidase digestion, and finally 1H NMR revealed the presence of L-fucose in α1,6-linkage to an α1,6-mannose of oligomannosidic N-glycans. The substitution by α1,6-mannose of α1,2-mannosyl residues of the canonical precursor structure was yet another hitherto unknown modification. No indication for the occurrence of yet other modifications, e.g. bisecting N-acetylglucosamine, was seen. Besides fucosylated N-glycans, short O-linked mannan chains substituted with fucose were present on chanterelle proteins. Although undiscovered so far, L-fucose appears to represent a prominent feature of protein-linked glycans in the fungal kingdom.
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    Journal Title
    Journal of Biological Chemistry
    Volume
    286
    Issue
    8
    DOI
    https://doi.org/10.1074/jbc.M110.191304
    Copyright Statement
    This research was originally published in Journal of Biological Chemistry (JBC). Grass et al, Discovery and structural characterization of fucosylated oligomannosidic N-glycans in mushrooms, Journal of Biological Chemistry (JBC) 2011; 286: 5977-5984 Copyright the American Society for Biochemistry and Molecular Biology. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitve version.
    Subject
    Chemical sciences
    Biological sciences
    Biochemistry and cell biology not elsewhere classified
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/339728
    Collection
    • Journal articles

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