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dc.contributor.authorFarag, Amroen_US
dc.contributor.authorVaquette, Cedrycken_US
dc.contributor.authorHutmacher, Dietmar W.en_US
dc.contributor.authorBartold, P. M.en_US
dc.contributor.authorIvanovski, Sasoen_US
dc.description.abstractDecellularized tissue-engineered constructs have the potential to promote regeneration by providing a biomimetic extracellular matrix that directs tissue-specific regeneration when implanted in situ. Recently, the use of cell sheets has shown promising results in promoting periodontal regeneration. Here, we describe the fabrication of decellularized periodontal cell sheets with intact extracellular matrix structural and biological properties. Melt electro spun polycaprolactone (PCL) scaffolds are used as a carrier for the inherently fragile cell sheets, to provide support during the processes of decellularization. An optimized decellularization method is outlined using perfusion with a combination of NH4OH and Triton X-100 together with a DNase treatment step for DNA removal. The maintenance of extracellular matrix structural and biological integrity is important, and here we describe the assessment of these properties using immunostaining for extracellular matrix proteins and ELISA for growth factor quantification.en_US
dc.publisherSpringer New Yorken_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofbooktitleOral Biology: Molecular Techniques and Applications, 2nd editionen_US
dc.subject.fieldofresearchMolecular Medicineen_US
dc.titleFabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructsen_US
dc.typeBook chapteren_US
dc.type.descriptionB1 - Book Chapters (HERDC)en_US
dc.type.codeB - Book Chaptersen_US
gro.facultyGriffith Health, School of Medical Scienceen_US
gro.hasfulltextNo Full Text

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