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dc.contributor.authorFarag, Amro
dc.contributor.authorVaquette, Cedryck
dc.contributor.authorHutmacher, Dietmar W
dc.contributor.authorBartold, P Mark
dc.contributor.authorIvanovski, Saso
dc.contributor.editorSeymour, GJ
dc.contributor.editorCullinan, MP
dc.contributor.editorHeng, NCK
dc.description.abstractDecellularized tissue-engineered constructs have the potential to promote regeneration by providing a biomimetic extracellular matrix that directs tissue-specific regeneration when implanted in situ. Recently, the use of cell sheets has shown promising results in promoting periodontal regeneration. Here, we describe the fabrication of decellularized periodontal cell sheets with intact extracellular matrix structural and biological properties. Melt electro spun polycaprolactone (PCL) scaffolds are used as a carrier for the inherently fragile cell sheets, to provide support during the processes of decellularization. An optimized decellularization method is outlined using perfusion with a combination of NH4OH and Triton X-100 together with a DNase treatment step for DNA removal. The maintenance of extracellular matrix structural and biological integrity is important, and here we describe the assessment of these properties using immunostaining for extracellular matrix proteins and ELISA for growth factor quantification.
dc.publisherSpringer New York
dc.publisher.placeUnited States
dc.relation.ispartofbooktitleOral Biology: Molecular Techniques and Applications, 2nd edition
dc.subject.fieldofresearchMolecular Medicine
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchOther Chemical Sciences
dc.titleFabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructs
dc.typeBook chapter
dc.type.descriptionB1 - Chapters
dc.type.codeB - Book Chapters
gro.facultyGriffith Health, School of Medical Science
gro.hasfulltextNo Full Text
gro.griffith.authorIvanovski, Saso
gro.griffith.authorFarag, Amro
gro.griffith.authorVaquette, Cedryck
gro.griffith.authorHutmacher, Dietmar W.

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