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  • Quantum dot-based sensitive detection of disease specific exosome in serum

    Author(s)
    Boriachek, Kseniia
    Islam, Md Nazmul
    Gopalan, Vinod
    Lam, Alfred K
    Nam-Trung, Nguyen
    Shiddiky, Muhammad JA
    Griffith University Author(s)
    Lam, Alfred K.
    Gopalan, Vinod
    Nguyen, Nam-Trung
    Shiddiky, Muhammad J.
    Year published
    2017
    Metadata
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    Abstract
    Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related. Here, we used CdSe quantum dot (CdSeQD) functionalised-biotinylated HER-2 and FAM134B antibodies ...
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    Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related. Here, we used CdSe quantum dot (CdSeQD) functionalised-biotinylated HER-2 and FAM134B antibodies as breast and colon cancer markers. After magnetic washing and purification steps, acid dissolution of CdSeQDs and subsequent anodic stripping voltammetric quantification of Cd2+ were carried out at the bare glassy carbon working electrode. This method enabled sensitive detection of 100 exosomes per μL with a relative standard deviation (%RSD) of <5.5% in cancer cell lines and a small cohort of serum samples (n = 9) collected from patients with colorectal adenocarcinoma. We believe that our approach could potentially represent an effective bioassay for the quantification of disease-specific exosomes in clinical samples.
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    Journal Title
    Analyst
    Volume
    142
    Issue
    12
    DOI
    https://doi.org/10.1039/C7AN00672A
    Subject
    Analytical chemistry
    Electrochemistry
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/340394
    Collection
    • Journal articles

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