A micro optofluidic system for counting and size measurement of particles
Author(s)
Nam-Trung, Nguyen
Song, Chaolong
Griffith University Author(s)
Year published
2012
Metadata
Show full item recordAbstract
This paper reports the integration of a biconvex micro optofluidic lens into a flow cytometer. The complex optofluidic and microfluidic channel networks are integrated on a single chip. Flow cytometers are widely applied to environmental monitoring, industrial testing and biochemical studies. Integrating a fow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the ...
View more >This paper reports the integration of a biconvex micro optofluidic lens into a flow cytometer. The complex optofluidic and microfluidic channel networks are integrated on a single chip. Flow cytometers are widely applied to environmental monitoring, industrial testing and biochemical studies. Integrating a fow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this paper, we propose a micro optofluidic system using an optofuidic liquid lens to focus the light beam. Benefting from the smooth liquid-liquid lens interface and the refractive index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced owing to the small beam width at the beam waist. Compared to the macroscale systems and microscale systems with solid lenses, the device presents a more efficient and accurate performance on both counting and sizing of particles. The paper reports an analytical parametric study of the lens, followed by the experimental performance of the cytometer. The cytometer was able to detect and discriminate particles with different sizes.
View less >
View more >This paper reports the integration of a biconvex micro optofluidic lens into a flow cytometer. The complex optofluidic and microfluidic channel networks are integrated on a single chip. Flow cytometers are widely applied to environmental monitoring, industrial testing and biochemical studies. Integrating a fow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this paper, we propose a micro optofluidic system using an optofuidic liquid lens to focus the light beam. Benefting from the smooth liquid-liquid lens interface and the refractive index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced owing to the small beam width at the beam waist. Compared to the macroscale systems and microscale systems with solid lenses, the device presents a more efficient and accurate performance on both counting and sizing of particles. The paper reports an analytical parametric study of the lens, followed by the experimental performance of the cytometer. The cytometer was able to detect and discriminate particles with different sizes.
View less >
Conference Title
PROCEEDINGS OF THE ASME 9TH INTERNATIONAL CONFERENCE ON NANOCHANNELS, MICROCHANNELS AND MINICHANNELS 2011, VOL 2
Volume
2
Copyright Statement
Self-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
Subject
Engineering practice and education not elsewhere classified